Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu, P. R. China.
Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou, P. R. China.
Nutr Cancer. 2021;73(1):160-168. doi: 10.1080/01635581.2020.1737153. Epub 2020 Mar 17.
Gastric cancer is one of the most common malignant tumors in the world, and prevention through diet is one of the ways to control. thunb.(HCT) is a plant having medicine and food function, has many biological properties. However, the effect of food style on the anticancer activity of HCT is not clear. So, we investigate the effect of heat treatment on anticancer activity of HCT. HCT extracts (heated aerial stem, heated subterraneous stem, heated leaves defined as HAS, HSS, HL, respectively, and not heated defined as NAS, NSS, NL, respectively) were obtained, and their inhibited activity were detected by alamar blue assay. The cell apoptosis was detected by DAPI staining and flow cytometry analysis. Western blot was performed to test the expression of apoptotic related protein. HCT showed the anticancer activity in four human tumor cell lines. Interestingly, heat treatment could increase the anticancer activity. In SCG-7901 cells, heat treatment increased anticancer activity of AS by 2-14 folds and induced apoptosis through regulating the intrinsic signaling pathways. Intriguingly, the caspase nine specific inhibitor blocked AS-reduced cell viability. Heat treatment increased the anticancer activity of HCT, and can be used as a dietary style for prevention of gastric cancer.
胃癌是世界上最常见的恶性肿瘤之一，通过饮食预防是控制胃癌的方法之一。旋覆花（HCT）是一种兼具药用和食用功能的植物，具有多种生物学特性。然而，食物方式对 HCT 抗癌活性的影响尚不清楚。因此，我们研究了热疗对 HCT 抗癌活性的影响。提取 HCT 提取物（加热地上茎、加热地下茎、加热叶片分别定义为 HAS、HSS、HL，未加热定义为 NAS、NSS、NL），通过 alamar blue 测定法检测其抑制活性。通过 DAPI 染色和流式细胞术分析检测细胞凋亡。通过 Western blot 检测凋亡相关蛋白的表达。HCT 在四种人肿瘤细胞系中表现出抗癌活性。有趣的是，热疗可以增加抗癌活性。在 SCG-7901 细胞中，热疗使 AS 的抗癌活性增加了 2-14 倍，并通过调节内在信号通路诱导细胞凋亡。有趣的是，半胱天冬酶 9 特异性抑制剂阻断了 AS 降低的细胞活力。热疗增加了 HCT 的抗癌活性，可作为预防胃癌的饮食方式。
