Department of Biotechnology, Institute of Biotechnology, Life and Applied Sciences, Yeungnam University, Gyeongsan, Gyeongbuk, 38541, Republic of Korea.
Department of Biology, College of Natural Sciences, Kyungpook National University, Buk-Gu, Daegu, 41566, Republic of Korea.
Appl Biochem Biotechnol. 2023 May;195(5):3027-3046. doi: 10.1007/s12010-022-04241-8. Epub 2022 Dec 10.
The biological activities of Houttuynia cordata (H. cordata) fermented with Aureobasidium pullulans (A. pullulans) was investigated for human skin keratinocyte-induced chemical and photo oxidations. In this research, HO/UVA-induced HaCaT cell lines were treated with H. cordata water/ethanol extracts (HCW/HCE) and fermented with A. pullulans water/ethanol extracts (HCFW/HCFE). A. pullulans fermented with H. cordata (HCFW) increased in 5.4-folds of total polyphenol (HCFW 46.89 mg GAE/extract g), and 2.3-folds in flavonoids (HCFW 53.80 mg GAE/extract g) compared with water extracts of H. cordata (HCW). Further, no significant cytotoxicity for HaCaT cells showed by all the extracts of H. cordata fermented with A. pullulans. HCFW extracts have significantly lowered inflammation factors such as COX-2 and Hsp70 proteins in oxidative stressed HaCaT cells induced by HO and UVA treatments. All H. cordata extracts significantly downregulated gene expression involved in oxidative stress and inflammation factors, including IL-1β, IL-6, COX-2, TNF-α, NF-κB, and MMP-1 in the HO/UVA-treated HaCaT cells. However, keratin-1 gene expression in the UVA-treated HaCaT cells was increased in twofolds by HCFW extracts. Further, A. pullulans fermented H. cordata extracts (HCFW/HCFE) reduced the genes involved in oxidative stresses more effectively than those of H. cordata extract only. Overall, the polyphenol-rich extracts of H. cordata fermented with A. pullulans showed synergistic protective effects for human epidermal keratinocytes to prevent photoaging and intrinsic aging by anti-oxidation and anti-inflammatory functions.
采用出芽短梗霉（ Aureobasidium pullulans ）发酵鱼腥草得到的生物活性产物对人皮肤角质形成细胞诱导的化学和光氧化的影响。在这项研究中，HO/UVA 诱导的 HaCaT 细胞系用鱼腥草水/乙醇提取物（HCW/HCE）和出芽短梗霉水/乙醇提取物（HCFW/HCFE）处理。与鱼腥草水提取物（HCW）相比，出芽短梗霉发酵鱼腥草（HCFW）的总多酚增加了 5.4 倍（HCFW 为 46.89 mg GAE/提取物 g），类黄酮增加了 2.3 倍（HCFW 为 53.80 mg GAE/提取物 g）。此外，所有用出芽短梗霉发酵的鱼腥草提取物对 HaCaT 细胞均无明显的细胞毒性。HCFW 提取物显著降低了 HO 和 UVA 处理诱导的氧化应激 HaCaT 细胞中 COX-2 和 Hsp70 等炎症因子的水平。所有鱼腥草提取物均显著下调了 HO/UVA 处理的 HaCaT 细胞中与氧化应激和炎症因子相关的基因表达，包括 IL-1β、IL-6、COX-2、TNF-α、NF-κB 和 MMP-1。然而，UVA 处理的 HaCaT 细胞中角蛋白 1 基因的表达在 HCFW 提取物的作用下增加了两倍。此外，与仅用鱼腥草提取物相比，出芽短梗霉发酵鱼腥草提取物（HCFW/HCFE）更有效地降低了与氧化应激相关的基因表达。总的来说，富含多酚的鱼腥草经出芽短梗霉发酵后，通过抗氧化和抗炎作用，为人类表皮角质形成细胞提供了协同的保护作用，以预防光老化和内在老化。
