Guo Huimin, Richel Aurore, Hao Yuqiong, Fan Xin, Everaert Nadia, Yang Xiushi, Ren Guixing
Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing China.
Gembloux Agro-Bio Tech University of Liège Gembloux Belgium.
Food Sci Nutr. 2020 Jan 27;8(3):1415-1422. doi: 10.1002/fsn3.1423. eCollection 2020 Mar.
Quinoa protein has been paid more and more attention because of its nutritional properties and beneficial effects. With the development of bioinformatics, bioactive peptide database and computer-assisted simulation provide an efficient and time-saving method for the theoretical estimation of potential bioactivities of protein. Therefore, the potential of quinoa protein sequences for releasing bioactive peptides was evaluated using the BIOPEP database, which revealed that quinoa protein, especially globulin, is a potential source of peptides with dipeptidyl peptidase-IV (DPP-IV) and angiotensin-I-converting enzyme (ACE) inhibitory activities. Three plant proteases, namely papain, ficin, and stem bromelain, were employed for the in silico proteolysis of quinoa protein. Furthermore, four tripeptides (MAF, NMF, HPF, and MCG) were screened as novel promising bioactive peptides by PeptideRanker. The bioactivities of selected peptides were confirmed using chemical synthesis and in vitro assay. The present work suggests that quinoa protein can serve as a good source of bioactive peptides, and in silico approach can provide theoretical assistance for investigation and production of functional peptides.
藜麦蛋白因其营养特性和有益作用而受到越来越多的关注。随着生物信息学的发展，生物活性肽数据库和计算机辅助模拟为蛋白质潜在生物活性的理论评估提供了一种高效且省时的方法。因此，利用BIOPEP数据库评估了藜麦蛋白序列释放生物活性肽的潜力，结果表明藜麦蛋白，尤其是球蛋白，是具有二肽基肽酶-IV（DPP-IV）和血管紧张素-I转换酶（ACE）抑制活性的肽的潜在来源。使用三种植物蛋白酶，即木瓜蛋白酶、无花果蛋白酶和菠萝蛋白酶，对藜麦蛋白进行了计算机模拟蛋白水解。此外，通过PeptideRanker筛选出四种三肽（MAF、NMF、HPF和MCG）作为新型有前景的生物活性肽。使用化学合成和体外试验证实了所选肽段的生物活性。目前的研究表明，藜麦蛋白可以作为生物活性肽良好来源，计算机模拟方法可为功能性肽的研究和生产提供理论帮助。
