Espersen G T, Ernst E, Christiansen O B, Jersild C, Grunnet N
Regional Center for Blood Transfusion and Clinical Immunology, Aalborg Hospital, Denmark.
Vox Sang. 1988;55(4):218-21. doi: 10.1111/j.1423-0410.1988.tb04700.x.
Platelet concentrates, irradiated with 15 Gy and stored for 5 days at room temperature under standardized conditions, were evaluated by in vitro tests and electron microscopy, in a paired study with nonirradiated platelets from the same concentrates, to investigate their usefulness for transfusion. The results showed no significant differences between the two groups in pH, pO2, pCO2, in vitro platelet aggregation, LDH, beta-thromboglobulin and thromboxane-B2. Examination by electron microscopy showed a higher degree of degranulation in the 5-day-old platelets but no certain difference between irradiated versus nonirradiated platelets. On the basis of satisfactory in vitro storage properties, platelet concentrates can be stored for 5 days in PL-1240 bags after irradiation. However, we recommend irradiation just before transfusion whenever possible.
在一项配对研究中，将浓缩血小板用15 Gy照射，并在标准化条件下于室温储存5天，然后通过体外试验和电子显微镜对其进行评估，同时与来自相同浓缩物的未照射血小板进行比较，以研究其输血的有效性。结果显示，两组在pH值、氧分压、二氧化碳分压、体外血小板聚集、乳酸脱氢酶、β-血小板球蛋白和血栓素B2方面没有显著差异。电子显微镜检查显示，储存5天的血小板脱颗粒程度更高，但照射血小板与未照射血小板之间没有明显差异。基于令人满意的体外储存特性，浓缩血小板在照射后可在PL-1240袋中储存5天。然而，我们建议尽可能在输血前即刻进行照射。
