Lown J A, Johnson W, Ivey J G
Department of Haematology, Royal Perth Hospital, Western Australia.
Vox Sang. 1988;55(4):229-32. doi: 10.1111/j.1423-0410.1988.tb04702.x.
For 18 months in this laboratory the manual polybrene technique (MP) has been used as the only crossmatching procedure preceded or accompanied by an antibody screen comprising two-stage papain and LISS antiglobulin techniques. There were 17,161 requests representing 43,006 blood units crossmatched and 20,841 units transfused. Non-specific reactivity with the MP required use of an antiglobulin crossmatch in approximately 0.2% of patient samples. Heparin in excess of 20 IU/ml reduced polybrene aggregation of red cells necessitating an antiglobulin crossmatch for 2 patients. Of 288 antibodies detected 20 reacted exclusively by MP compared with 18 by the papain procedure. The data supported the use of MP as an alternative to enzymes in antibody screening protocols. The polybrene technique was found to be a superior abbreviated crossmatch compared with the immediate spin technique and was applicable to all patients including those with known antibodies.
在本实验室，十八个月来手工聚凝胺技术（MP）一直被用作唯一的交叉配血程序，在此之前或同时进行抗体筛查，抗体筛查采用两阶段木瓜蛋白酶和低离子强度溶液抗球蛋白技术。共有17161次申请，涉及43006个单位血液的交叉配血以及20841个单位血液的输注。在大约0.2%的患者样本中，与MP存在非特异性反应，需要使用抗球蛋白交叉配血。肝素浓度超过20 IU/ml会降低红细胞的聚凝胺凝集作用，有2例患者因此需要进行抗球蛋白交叉配血。在检测出的288种抗体中，有20种仅通过MP反应，而通过木瓜蛋白酶程序反应的有18种。这些数据支持将MP用作抗体筛查方案中酶法的替代方法。结果发现，聚凝胺技术与立即离心技术相比，是一种更优的简化交叉配血方法，适用于所有患者，包括已知有抗体的患者。
