Department of Emergency, Disaster, and Critical Care Medicine, Showa University of Medicine, Tokyo, Japan.
PLoS One. 2020 Mar 17;15(3):e0230533. doi: 10.1371/journal.pone.0230533. eCollection 2020.
As part of a verification model of antibiotic therapy in cranioplasty, we evaluated the impregnation efficiency of interporous calcium phosphate materials with saline under atmospheric pressure and compared it to the efficiency of using the decompression method established by the Japanese Industrial Standard, under which pressure is reduced by 10 kPa. Five types of material formed in 1 mL cubes were selected as test samples: two consisting of hydroxyapatite (HAp) with 85% and 55% porosity and three of β-tricalcium phosphate (β-TCP) with 75%, 67%, and 57% porosity. All test samples showed an impregnation ratio of more than 70%, except for the HAp sample with 55% porosity, which had a ratio of approximately 50%. These high ratios were achieved at only 15 min. The impregnation effects were likely dependent on porosity and were independent of base material, either HAp or β-TCP. Obtaining sufficient impregnation and antimicrobial efficacy in materials with low porosity, which are commonly used in cranioplasty, would require an increased volume of antibiotics rather than increased duration of impregnation. Our findings will enable the simple preparation of drug-impregnated calcium phosphate materials, even in operating rooms not equipped with a large decompression device.
作为颅骨修复术中抗生素治疗验证模型的一部分，我们评估了在大气压力下用生理盐水对多孔磷酸钙材料进行浸渍的效率，并将其与日本工业标准规定的减压方法的效率进行了比较，减压方法将压力降低 10 kPa。选择了 5 种在 1 mL 立方体内形成的材料作为测试样品：两种由 85%和 55%孔隙率的羟基磷灰石（HAp）组成，三种由 75%、67%和 57%孔隙率的β-磷酸三钙（β-TCP）组成。所有测试样品的浸渍率均超过 70%，除了孔隙率为 55%的 HAp 样品，其浸渍率约为 50%。这些高比例仅在 15 分钟内实现。浸渍效果可能取决于孔隙率，而与基础材料（HAp 或 β-TCP）无关。在颅骨修复术中常用的低孔隙率材料中获得足够的浸渍和抗菌效果可能需要增加抗生素的体积，而不是增加浸渍时间。我们的发现将使简单制备载药磷酸钙材料成为可能，即使在没有大型减压设备的手术室也可以实现。
