Biomedical Center, Chromatin Proteomics Group, Department of Molecular Biology, Faculty of Medicine, Ludwig-Maximilians-Universität München, Großhaderner Strasse 9, 82152 Planegg-Martinsried, Germany.
Biomedical Center, Bioinformatics Core Facility, Ludwig-Maximilians-Universität München, Großhaderner Strasse 9, 82152 Planegg-Martinsried, Germany.
Nucleic Acids Res. 2020 May 7;48(8):4161-4178. doi: 10.1093/nar/gkaa145.
During interphase centromeres often coalesce into a small number of chromocenters, which can be visualized as distinct, DAPI dense nuclear domains. Intact chromocenters play a major role in maintaining genome stability as they stabilize the transcriptionally silent state of repetitive DNA while ensuring centromere function. Despite its biological importance, relatively little is known about the molecular composition of the chromocenter or the processes that mediate chromocenter formation and maintenance. To provide a deeper molecular insight into the composition of the chromocenter and to demonstrate the usefulness of proximity-based biotinylation as a tool to investigate those questions, we performed super resolution microscopy and proximity-based biotinylation experiments of three distinct proteins associated with the chromocenter in Drosophila. Our work revealed an intricate internal architecture of the chromocenter suggesting a complex multilayered structure of this intranuclear domain.
在间期，着丝粒通常会凝聚成少数几个染色中心，这些中心可以被视为明显的、DAPI 密集的核域。完整的染色中心在维持基因组稳定性方面起着重要作用，因为它们稳定了重复 DNA 的转录沉默状态，同时确保了着丝粒的功能。尽管其具有重要的生物学意义，但关于染色中心的分子组成或介导染色中心形成和维持的过程，人们知之甚少。为了更深入地了解染色中心的组成，并展示基于邻近性的生物素化作为研究这些问题的工具的有用性，我们对与果蝇中染色中心相关的三种不同蛋白质进行了超分辨率显微镜和基于邻近性的生物素化实验。我们的工作揭示了染色中心复杂的内部结构，表明这个核内域具有复杂的多层次结构。
