Department of Cell Biology, Yale School of Medicine, New Haven, CT.
J Cell Biol. 2020 May 4;219(5). doi: 10.1083/jcb.202001161.
A single particle cryo-EM reconstruction of an ∼160-kD N-terminal fragment of the lipid transport protein VPS13 reveals an ∼160-Å long channel lined with hydrophobic residues suitable for solubilizing multiple lipid fatty acid moieties. The structure suggests that VPS13 and related proteins, like the autophagy protein ATG2, can act as bridges between organelle membranes to allow bulk lipid flow between organelles.
一个约 160kD 的脂质转运蛋白 VPS13 的 N 端片段的单颗粒 cryo-EM 重建揭示了一个约 160-Å 长的通道，由疏水性残基排列，适合溶解多个脂质脂肪酸部分。该结构表明，VPS13 和相关蛋白，如自噬蛋白 ATG2，可以作为细胞器膜之间的桥梁，允许细胞器之间的大量脂质流动。
