Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, Poland.
Institute of Biomedical Engineering, Bialystok University of Technology, Wiejska 45C, 15-351 Bialystok, Poland.
Int J Mol Sci. 2020 Mar 13;21(6):1979. doi: 10.3390/ijms21061979.
Saliva plays a crucial role in oral cavity. In addition to its buffering and moisturizing properties, saliva fulfills many biofunctional requirements, including antibacterial activity that is essential to assure proper oral microbiota growth. Due to numerous extra- and intra-systemic factors, there are many disorders of its secretion, leading to oral dryness. Saliva substitutes used in such situations must meet many demands. This study was design to evaluate the effect of core-shell magnetic nanoparticles (MNPs) adding (gold-coated and aminosilane-coated nanoparticles NPs) on antimicrobial (microorganism adhesion, biofilm formation), rheological (viscosity, viscoelasticity) and physicochemical (pH, surface tension, conductivity) properties of three commercially available saliva formulations. Upon the addition of NPs (20 µg/mL), antibacterial activity of artificial saliva was found to increase against tested microorganisms by 20% to 50%. NPs, especially gold-coated ones, decrease the adhesion of Gram-positive and fungal cells by 65% and Gram-negative bacteria cells by 45%. Moreover, the addition of NPs strengthened the antimicrobial properties of tested artificial saliva, without influencing their rheological and physicochemical properties, which stay within the range characterizing the natural saliva collected from healthy subjects.
唾液在口腔中起着至关重要的作用。除了具有缓冲和保湿特性外，唾液还满足许多生物功能要求，包括抗菌活性，这对于确保适当的口腔微生物群落生长至关重要。由于许多内外系统因素，唾液的分泌会出现许多紊乱，导致口腔干燥。在这种情况下使用的唾液替代品必须满足许多要求。本研究旨在评估添加核壳型磁性纳米颗粒（MNPs）（金包覆和氨基硅烷包覆纳米颗粒 NPs）对三种市售唾液制剂的抗菌（微生物黏附、生物膜形成）、流变学（黏度、黏弹性）和物理化学（pH 值、表面张力、电导率）性质的影响。加入 NPs（20μg/mL）后，发现人工唾液对测试微生物的抗菌活性提高了 20%至 50%。NPs，尤其是金包覆的 NPs，可使革兰氏阳性和真菌细胞的黏附减少 65%，革兰氏阴性细菌细胞的黏附减少 45%。此外，加入 NPs 增强了测试人工唾液的抗菌性能，而不会影响其流变学和物理化学性质，这些性质仍在从健康受试者收集的天然唾液的特征范围内。
