A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
Department of Reproduction Management, Leibniz-Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany.
Cells. 2020 Mar 14;9(3):712. doi: 10.3390/cells9030712.
Electron microscopic study of cardiomyocytes taken from healthy Wistar and OXYS rats and naked mole rats () revealed mitochondria in nuclei that lacked part of the nuclear envelope. The direct interaction of mitochondria with nucleoplasm is shown. The statistical analysis of the occurrence of mitochondria in cardiomyocyte nuclei showed that the percentage of nuclei with mitochondria was roughly around 1%, and did not show age and species dependency. Confocal microscopy of normal rat cardiac myocytes revealed a branched mitochondrial network in the vicinity of nuclei with an organization different than that of interfibrillar mitochondria. This mitochondrial network was energetically functional because it carried the membrane potential that responded by oscillatory mode after photodynamic challenge. We suggest that the presence of functional mitochondria in the nucleus is not only a consequence of certain pathologies but rather represents a normal biological phenomenon involved in mitochondrial/nuclear interactions.
电子显微镜研究取自健康的 Wistar 和 OXYS 大鼠和裸鼹鼠（）的心肌细胞，揭示了细胞核内的线粒体缺乏部分核膜。显示了线粒体与核质的直接相互作用。对心肌细胞核中线粒体出现的统计分析表明，具有线粒体的细胞核的百分比大致在 1%左右，并且不显示年龄和物种依赖性。正常大鼠心肌细胞的共聚焦显微镜显示，细胞核附近存在分支的线粒体网络，其组织与纤维间线粒体不同。这个线粒体网络具有能量功能，因为它携带膜电位，在光动力挑战后以振荡模式响应。我们认为，细胞核中存在功能线粒体不仅是某些病理的结果，而是代表了涉及线粒体/核相互作用的正常生物学现象。
