Susai Surraj, Chandrupatla Mrudula, Sivakoti Sumitra, Kusneniwa Govindrao N, Pyati Anand K
All India Institute of Medical Sciences, Department of Anatomy, Bibinagar, India.
All India Institute of Medical Sciences, Department of Pathology, Bibinagar, India.
Medeni Med J. 2025 Jun 26;40(2):93-100. doi: 10.4274/MMJ.galenos.2025.87120.
This study aimed to determine the whole free mitochondria in type-2 diabetic buccal epithelial cells using a supravital stain called "Janus Green B" and assess their behavior after exposure to near infrared light. The researchers observed microscopic mitochondrial load and its intracellular spatial behavior after exposure to near-infrared rays, bridging the gap in understanding mitochondrial orientation in diseases like diabetes. The aim of this research was to find out the quantitative involvement and electrostatic intracellular spatial patterns of whole mitochondria in diabetes.
Exfoliated buccal cell wet mounts, supravitally stained using Janus green, and excited using infrared rays, were observed using an advanced bright field Axiocam microscope, and the images of whole mitochondria within the cells were photographed and analyzed using the ZEN 2.0 cell sense software. The migration patterns of mitochondria were observed.
A quantitative decrease in mitochondria was noted in diabetic cells. Signatures of clumpy peripheral shifts in mitochondria were observed in diabetic buccal cells post radiation.
Advanced glycation end products of diabetes combined with oxidative stressors influenced the free mitochondria to clump peripherally and produce a characteristic signature. The decreased mitochondrial load contributed additional evidence to the reduced respiratory capacity of cells, which forced mitochondria to emit a detectable signature when irradiated.
本研究旨在使用一种名为“健那绿B”的超活染色剂来测定2型糖尿病患者颊黏膜上皮细胞中的完整游离线粒体，并评估其在近红外光照射后的行为。研究人员观察了近红外线照射后线粒体的微观负载及其细胞内空间行为，填补了在糖尿病等疾病中对线粒体定位理解的空白。本研究的目的是找出糖尿病中完整线粒体的定量参与情况和静电细胞内空间模式。
使用健那绿进行超活染色并经红外线激发的脱落颊细胞湿片，通过先进的明场Axiocam显微镜进行观察，并使用ZEN 2.0细胞感知软件对细胞内完整线粒体的图像进行拍照和分析。观察线粒体的迁移模式。
糖尿病细胞中线粒体数量出现定量减少。在辐射后的糖尿病颊细胞中观察到线粒体呈团块状向周边移位的特征。
糖尿病的晚期糖基化终产物与氧化应激源共同作用，影响游离线粒体向周边聚集并产生特征性信号。线粒体负载的减少为细胞呼吸能力下降提供了额外证据，这使得线粒体在受到照射时发出可检测的信号。
