College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150001, China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, 150001, China.
Virol J. 2020 Mar 17;17(1):39. doi: 10.1186/s12985-020-01312-2.
Pseudorabies virus (PRV) is an important pathogen that threatens the global swine industry. Currently, there is no effective drug that can clinically prevent or treat PRV infections. Isobavachalcone (IBC), a natural chalcone compound derived from Psoralea corylifolia, displays multiple biological activities, such as antibacterial, antifungal, and anticancer activities. Recently, it was found that IBC exhibited antiviral activity against an RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), in vitro. In the current study, we further demonstrated for the first time that IBC has a strong inhibitory effect on PRV. Through a viral luciferase expression assay, we showed that the inhibition step occurs mainly in the late stage of viral replication. Finally, via a cell-to-cell fusion assay, we demonstrated that IBC inhibits PRV by blocking virus-mediated cell fusion. Thus, IBC may be a candidate for further therapeutic evaluation against PRV infection in vivo.
伪狂犬病毒（PRV）是一种重要的病原体，威胁着全球养猪业。目前，临床上尚无有效的药物可以预防或治疗 PRV 感染。异甘草素（IBC）是一种从补骨脂中提取的天然查尔酮化合物，具有多种生物学活性，如抗菌、抗真菌和抗癌活性。最近，人们发现 IBC 在体外对 RNA 病毒猪繁殖与呼吸综合征病毒（PRRSV）具有抗病毒活性。在本研究中，我们首次进一步证明 IBC 对 PRV 具有很强的抑制作用。通过病毒荧光素酶表达试验，我们表明抑制步骤主要发生在病毒复制的晚期。最后，通过细胞融合试验，我们证明 IBC 通过阻断病毒介导的细胞融合来抑制 PRV。因此，IBC 可能是进一步评估其在体内抗 PRV 感染的候选药物。
