State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Viruses. 2023 Jan 5;15(1):170. doi: 10.3390/v15010170.
Pseudorabies virus (PRV) generally infects pigs and threatens the pig industry. However, recently we have isolated a PRV strain designated hSD-1/2019 from infected humans. In this study, we compared the complete genome sequence of hSD-1/2019 with those of pig-originated PRV strains. Sequence alignments revealed that the genome sequence of hSD-1/2019 was highly homologous to those of the porcine PRV strains. Phylogenetic analyses found that hSD-1/2019 was the closest related to porcine PRV endemic strains in China, particularly the variant strains circulating recently. We also showed that the glycoproteins important for the multiplication and pathogenesis of hSD-1/2019 were highly similar to those of the pig endemic strains. Diversifying selection analyses revealed that hSD-1/2019 and pig variant strains are under diversifying selection. Recombination analysis indicated that hSD-1/2019 was a recombinant of several PRV variant strains and an earlier PRV classic strain. Finally, we found that both human and pig-originated PRV strains could induce cytopathic effects in cells from humans, pigs, and mice, but only the human PRV and pig-variant PRV formed large syncytia in human cell lines. The data presented in this study contribute to our understanding of the molecular basis for the pathogenesis of human PRV from a genomic aspect.
伪狂犬病毒（PRV）通常感染猪，对猪产业构成威胁。然而，最近我们从感染的人类中分离到一株 PRV 毒株，命名为 hSD-1/2019。在本研究中，我们比较了 hSD-1/2019 的全基因组序列与猪源 PRV 毒株的序列。序列比对显示，hSD-1/2019 的基因组序列与猪源 PRV 毒株高度同源。系统进化分析发现，hSD-1/2019 与中国流行的猪源 PRV 地方性毒株最为接近，尤其是最近流行的变异株。我们还表明，hSD-1/2019 对病毒增殖和发病机制至关重要的糖蛋白与猪源地方性毒株高度相似。多样化选择分析显示，hSD-1/2019 和猪变异株处于多样化选择压力下。重组分析表明，hSD-1/2019 是几种 PRV 变异株和早期 PRV 经典株的重组体。最后，我们发现人和猪源 PRV 株均可在人、猪和鼠源细胞中诱导细胞病变效应，但只有人源 PRV 和猪变异株在人源细胞系中形成大的合胞体。本研究的数据从基因组角度为我们理解人源 PRV 的发病机制的分子基础提供了帮助。
