鱼抗菌肽 1 对猪伪狂犬病病毒的体内外抗病毒活性。

Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo.

机构信息

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.

Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China.

出版信息

Virol J. 2019 Jul 31;16(1):95. doi: 10.1186/s12985-019-1199-4.

BACKGROUND

Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation.

METHODS

In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important swine-origin pathogenic viruses by TCID assay. Plaque reduction assay and cell apoptosis assay were also used to test the activity of the peptides. Protection effect of piscidin against pseudorabies virus (PRV) was also examined in mouse model.

RESULTS

Piscidin (piscidin 1), caerin (caerin 1.1) and maculatin (maculatin 1.1) could inhibit PRV by direct interaction with the virus particles in a dose-dependent manner and they could also protect the cells from PRV-induced apoptosis. Among the peptides tested, piscidin showed the strongest activity against PRV. Moreover, in vivo assay showed that piscidin can reduce the mortality of mice infected with PRV.

CONCLUSION

In vitro and in vivo experiments indicate that piscidin has antiviral activity against PRV.

背景

猪源病毒的广泛传播可能会给养猪业造成巨大的经济损失。因此，需要开发新型抗病毒药物来控制这种情况。

方法

本研究通过 TCID 法评估了五种广谱抗菌肽（AMPs）对几种重要猪源致病病毒的活性。噬菌斑减少试验和细胞凋亡试验也用于测试肽的活性。还在小鼠模型中检测了鱼精蛋白对伪狂犬病病毒（PRV）的保护作用。

结果

鱼精蛋白（鱼精蛋白 1）、钙蛋白（钙蛋白 1.1）和马库拉丁（马库拉丁 1.1）可以通过与病毒颗粒直接相互作用，以剂量依赖的方式抑制 PRV，并且可以保护细胞免受 PRV 诱导的凋亡。在测试的肽中，鱼精蛋白对 PRV 表现出最强的活性。此外，体内实验表明，鱼精蛋白可以降低感染 PRV 的小鼠的死亡率。