Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul, 05029, South Korea.
Korea Institute of Dermatological Science, GeneCellPharm Corporation, 375 Munjeong 2(i)-dong, Songpa-gu, Seoul, 05836, South Korea.
Sci Rep. 2020 Mar 17;10(1):4887. doi: 10.1038/s41598-020-61824-3.
Cellular metabolism is one of the crucial factors to regulate epigenetic landscape in various cells including immune cells, embryonic stem cells and hair follicle stem cells. Dermal papilla cells (DP) interact with epithelial stem cells to orchestrate hair formation. Here we show that active DP exhibit robust aerobic glycolysis. We observed decrease of signature genes associated with hair induction by DP in presence of low glucose (2 mM) and glycolysis inhibitors. Moreover, hair shaft elongation was attenuated by glycolysis inhibitors. Interestingly, excessive glucose is able to increase the expression of hair inductive genes and elongation of hair shaft. We also observed glycolysis-mediated histone acetylation is increased and chemical inhibition of acetyltransferase reduces expression of the signature genes associated with hair induction in active DP. These results suggest that glucose metabolism is required for expression of signature genes associated with hair induction. This finding may be beneficial for establishing and maintaining of active DP to generate hair follicle in vitro.
细胞代谢是调节各种细胞（包括免疫细胞、胚胎干细胞和毛囊干细胞）表观遗传景观的关键因素之一。真皮乳头细胞（DP）与上皮干细胞相互作用以协调毛发形成。在这里，我们表明活跃的 DP 表现出强大的有氧糖酵解。我们观察到在低葡萄糖（2mM）和糖酵解抑制剂存在下，与 DP 诱导毛发相关的特征基因表达减少。此外，糖酵解抑制剂会减弱毛干伸长。有趣的是，过多的葡萄糖能够增加诱导毛发生长的基因表达和毛干伸长。我们还观察到糖酵解介导的组蛋白乙酰化增加，并且乙酰转移酶的化学抑制降低了活跃 DP 中与诱导毛发相关的特征基因的表达。这些结果表明，葡萄糖代谢是与诱导毛发相关的特征基因表达所必需的。这一发现可能有益于建立和维持活跃的 DP 以在体外产生毛囊。
