Photolo Mampolelo M, Mavumengwana Vuyo, Sitole Lungile, Tlou Matsobane G
Department of Biochemistry, Faculty of Science, University of Johannesburg, Auckland Park Campus, Johannesburg, South Africa.
DST-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg Campus, Cape Town, South Africa.
Int J Microbiol. 2020 Feb 18;2020:9483670. doi: 10.1155/2020/9483670. eCollection 2020.
This study reports on the isolation and identification of MAMP 4754 from the seeds of the medicinal plant, for the purposes of investigating antimicrobial and antioxidant activities from this endophyte. The strain identity was confirmed by 16S rRNA-based phylogeny and Scanning Electron Microscopy (SEM). Ethyl acetate and chloroform (1 : 1 v/v) extracts from the endophyte were tested for antimicrobial and antioxidant activity on a total of 7 bacterial species (3 Gram-positive and 4 Gram-negative) using the standard Minimum Inhibitory Concentration (MIC) protocol and Quantitative Radical Scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The MICs were recorded at 250 g/mL for ATCC 19659, ATCC 1076, ATCC1053, and 62.5 g/mL for ATCC 13182 and ATCC 21293, while an IC of 5.65 g/mL was recorded with the DPPH assay. Qualitative phytochemical analysis was positive for alkaloids, flavonoids, and steroids. Gas chromatography/mass spectrometry (GC/MS) analysis revealed the presence of 9-octadecene, 2,4-dinitrophenyl acetate, and 2(5H)-furanone, which have been previously reported for the targeted activities. MAMP 4754 tested positive for antimicrobial and antioxidant activity and this is linked to the production of plant-derived secondary metabolites by this strain.
本研究报告了从药用植物种子中分离和鉴定MAMP 4754的过程，目的是研究这种内生菌的抗菌和抗氧化活性。通过基于16S rRNA的系统发育分析和扫描电子显微镜（SEM）确定了该菌株的身份。使用标准的最低抑菌浓度（MIC）方案，对该内生菌的乙酸乙酯和氯仿（1∶1 v/v）提取物针对总共7种细菌（3种革兰氏阳性菌和4种革兰氏阴性菌）进行了抗菌活性测试，并分别使用2,2-二苯基-1-苦基肼（DPPH）法进行了定量自由基清除活性测试。对于ATCC 19659、ATCC 1076、ATCC1053，MIC记录为250μg/mL，对于ATCC 13182和ATCC 21293，MIC记录为62.5μg/mL，而DPPH法测得的IC50为5.65μg/mL。定性植物化学分析表明存在生物碱、黄酮类化合物和甾体。气相色谱/质谱（GC/MS）分析显示存在9-十八碳烯、乙酸2,4-二硝基苯酯和2(5H)-呋喃酮，这些物质先前已被报道具有相关活性。MAMP 4754的抗菌和抗氧化活性测试呈阳性，这与该菌株产生植物源次生代谢产物有关。
