Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China.
Sci Rep. 2019 Jul 17;9(1):10389. doi: 10.1038/s41598-019-46863-9.
Dendrobium is known for its pharmacological actions including anti-cancer effect, anti-fatigue effect, gastric ulcer protective effect, and so on. At present, only studies on endophytic fungi of Dendrobium affecting the metabolites of host plants have been reported, very little research has been done on endophytic bacteria. In this study, we have demonstrated the great diversity of endophytic bacteria in 6 Dendrobium samples from different origins and cultivars. According to the results of the culture-independent method, the endophytic bacterial community in Dendrobium stems showed obvious different in the 6 samples and was influenced by origin and cultivar. Some bacteria including Ralstonia, Comamonas and Lelliottia were first detected in Dendrobium in this study. Based on the culture-dependent method, a total of 165 cultivable endophytic bacteria isolates were isolated from the sterilized Dendrobium stems, and were classified into 43 species according to the 16S rRNA gene sequence analysis. Moreover, 14 of the 43 strains showed antimicrobial activity against phytopathogen using the Kirby-Bauer method. Strain NA-HTong-7 (Bacillus megaterium, 99.12%) showed the highest antimicrobial activity. This study was the first comprehensive study on endophytic bacteria of Dendrobium from different origins and cultivars, which provides new insights into the endophytic bacteria from Dendrobium.
铁皮石斛以其药理作用而闻名，包括抗癌作用、抗疲劳作用、胃溃保护作用等。目前，仅报道了关于影响宿主植物代谢物的铁皮石斛内生真菌的研究，对内生细菌的研究很少。在这项研究中，我们展示了来自不同产地和品种的 6 个铁皮石斛样本中的内生细菌的巨大多样性。根据非培养方法的结果，铁皮石斛茎中的内生细菌群落在 6 个样本中表现出明显的不同，并且受到产地和品种的影响。在本研究中，首次在铁皮石斛中检测到了一些细菌，如 Ralstonia、Comamonas 和 Lelliottia。基于培养依赖的方法，从消毒的铁皮石斛茎中总共分离出了 165 株可培养的内生细菌分离株，并根据 16S rRNA 基因序列分析将其分为 43 个种。此外，用 Kirby-Bauer 法对 43 株中的 14 株进行了对植物病原菌的抗菌活性检测。菌株 NA-HTong-7（巨大芽孢杆菌，99.12%）表现出最高的抗菌活性。这项研究是对来自不同产地和品种的铁皮石斛内生细菌的首次综合研究，为铁皮石斛内生细菌提供了新的见解。
