Honarvar Fatemeh, Hojati Vida, Bakhtiari Nuredin, Vaezi Gholamhassan, Javan Mohammad
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Biochemistry, Faculty of Biological Science, North_Tehran Branch, Tehran, Iran.
Iran J Pharm Res. 2019 Fall;18(4):1978-1988. doi: 10.22037/ijpr.2019.112181.13582.
Neuronal survival in multiple sclerosis (MS) and other demyelinating diseases depends on the preservation of myelin and remyelination of axons. Myelin protection is the main purpose to decrease myelin damage in the central nervous system (CNS). Ursolic acid (UA) as a natural product in apple is suggested to protect neural cells. This study is the first to demonstrate an effect for UA on CNS myelin loss induced by cuprizone toxin. In the current study, we hypothesized that daily treatment with UA in drinking water (1 mg/mL) prevents myelin damage by 6 weeks administration of CPZ in mice pellet which lead to corpus callosum axonal demyelination. We assessed the myelin content and the number of myelinating cells in corpus callosum by FluoroMyelin and luxol fast blue staining as well as by immunostaining against MBP and Olig2. Our finding indicated that UA could decrease the extent of demyelination area and enhanced myelin stain intensity within CC and protected oligodendrocyte lineage cells against cuprizone toxin. We could conclude that myelinated structures could be protected by UA in corpus callosum, which provide favorable evidence for the possibility of application of UA in demyelinating diseases and traumatic injuries.
多发性硬化症（MS）和其他脱髓鞘疾病中的神经元存活取决于髓磷脂的保存和轴突的重新髓鞘化。髓磷脂保护是减少中枢神经系统（CNS）中髓磷脂损伤的主要目的。熊果酸（UA）作为苹果中的一种天然产物，被认为可以保护神经细胞。本研究首次证明了UA对由铜离子螯合剂毒素诱导的中枢神经系统髓鞘损失的作用。在当前研究中，我们假设在饮用水中每日给予UA（1mg/mL），通过在小鼠颗粒中给予6周的铜离子螯合剂（CPZ）来预防髓磷脂损伤，这会导致胼胝体轴突脱髓鞘。我们通过荧光髓磷脂和Luxol固蓝染色以及针对髓鞘碱性蛋白（MBP）和少突胶质细胞转录因子2（Olig2）的免疫染色来评估胼胝体中的髓磷脂含量和髓鞘形成细胞的数量。我们的研究结果表明，UA可以减少脱髓鞘区域的范围，增强胼胝体内髓磷脂染色强度，并保护少突胶质细胞谱系细胞免受铜离子螯合剂毒素的影响。我们可以得出结论，UA可以保护胼胝体中的髓鞘结构，这为UA在脱髓鞘疾病和创伤性损伤中的应用可能性提供了有利证据。
