Department of Clinical Pharmacology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
J Tradit Chin Med. 2019 Jun;39(3):324-331.
To investigate the radioprotective effect of tea polyphenols (TP 50) against radiation-induced organ and tissue damage.
Beagle dogs were exposed to a single acute dose of whole-body γ-radiation (3 Gy) and orally administered TP 50 (80 or 240 mg·kg－1·d－1) for 28 consecutive days. A hemogram was obtained from experimental dogs every other day for 42 d. At the end of the experiment, enzyme activities of the antioxidants superoxide-dismutase andglutathione peroxidase, serum levels of inflamma- tory cytokines (tumor necrosis factor-α, interleukin-1β, and interleukin-6), colony-forming units of bone marrow hematopoietic progenitor cells, andorgan coefficients were measured.
Dogs exposed to γ-radiation alone exhibited typical hematopoietic syndrome. In contrast, irradiated dogs that received TP 50 exhibited an improved blood profile with reduced leucopenia, thrombocytopenia (platelet counts), and reticulocyte levels. TP 50 also significantly elevated levels of the endogenous antioxidant enzyme superoxide-dismutase, reduced the increased levels of serum cytokine in response to radiation-induced toxicity, and increased colony-forming units of bone marrow hematopoietic progenitor cells. In addition, TP 50 repaired radiation-induced organ damage.
The current findings suggest that oral administration of TP 50 to beagle dogs effectively alleviated hematopoietic bone marrow dam- age induced by γ-radiation.
研究茶多酚（TP50）对辐射诱导的器官和组织损伤的放射防护作用。
Beagle 犬单次全身γ射线照射（3Gy），连续 28 天口服 TP50（80 或 240mg·kg－1·d－1）。实验犬每 2 天进行一次血常规检查，共 42 天。实验结束时，测定抗氧化酶超氧化物歧化酶和谷胱甘肽过氧化物酶的酶活性、血清炎症细胞因子（肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6）水平、骨髓造血祖细胞集落形成单位和器官系数。
单独接受γ射线照射的犬表现出典型的造血综合征。相比之下，接受 TP50 的照射犬的血液状况得到改善，白细胞减少症、血小板减少症（血小板计数）和网织红细胞水平降低。TP50 还显著提高了内源性抗氧化酶超氧化物歧化酶的水平，降低了血清细胞因子因辐射毒性而升高的水平，并增加了骨髓造血祖细胞集落形成单位。此外，TP50 修复了辐射诱导的器官损伤。
目前的研究结果表明，口服 TP50 可有效缓解γ 射线诱导的 Beagle 犬造血骨髓损伤。
