Department of Clinical Pharmacology and Pharmacy, Centre of Pharmacy, Chinese PLA General Hospital, Beijing, 100853, China.
Phytother Res. 2011 Dec;25(12):1761-9. doi: 10.1002/ptr.3483. Epub 2011 Mar 31.
The purpose of this study was to investigate the radioprotective effects of tea polyphenols (TPs) in various combinations against radiation-induced damage in mice. Mice were divided into different groups: non-irradiated control, irradiated control, amifostine (43.6 mg/kg, i.v. 30 min before irradiation; positive control) and various combinations of tea polyphenols in different doses. The radioprotective effect on the haematopoietic system, serum cytokines and endogenous antioxidant enzymes were studied. TP50, containing approximately 50% of (-)-epigallochatechin-3-gallate in addition to other catechins, showed the greatest radioprotective effect against radiation-induced changes in haematological parameters (red blood cell count, white blood cell count and haemoglobin), and maintained the spleen and thymus indices unchanged (spleen or thymus weight/body weight × 1000). Tea polyphenols also significantly decreased radiation-induced lipid peroxidation (malondialdehyde levels), elevated endogenous antioxidant enzymes (superoxide dismutase) and reduced the serum cytokines which were elevated in radiation-induced toxicity. This evidence shows the potential of tea polyphenols, particularly in the combination found in TP50, as radioprotectors in mice, especially regarding recovery of the haematopoietic system, antioxidant potential activity and reduction of inflammatory cytokines.
本研究旨在探讨茶多酚（TPs）在不同组合下对辐射诱导的小鼠损伤的放射防护作用。将小鼠分为不同组：非照射对照组、照射对照组、氨磷汀（43.6mg/kg，静脉注射，照射前 30 分钟；阳性对照）和不同剂量的各种组合的茶多酚。研究了对造血系统、血清细胞因子和内源性抗氧化酶的放射防护作用。TP50 含有约 50%的（-）-表没食子儿茶素-3-没食子酸酯，此外还含有其他儿茶素，对辐射引起的血液学参数变化（红细胞计数、白细胞计数和血红蛋白）具有最大的放射防护作用，并保持脾和胸腺指数不变（脾或胸腺重量/体重×1000）。茶多酚还显著降低了辐射诱导的脂质过氧化（丙二醛水平），提高了内源性抗氧化酶（超氧化物歧化酶）的水平，并降低了血清细胞因子，这些细胞因子在辐射诱导的毒性中升高。这些证据表明，茶多酚，特别是在 TP50 中发现的组合，具有作为小鼠放射保护剂的潜力，特别是在造血系统恢复、抗氧化潜能活性和减少炎症细胞因子方面。
