Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, Germany.
Spectral Service AG, Emil-Hoffmann-Strasse 33, D-50996 Köln, Germany.
Biomacromolecules. 2020 May 11;21(5):1929-1942. doi: 10.1021/acs.biomac.0c00123. Epub 2020 Mar 24.
A catalyst-free organosolv pulping process was applied to cup plant (, S), Miscanthus grass (, M), and the Paulownia tree (, P), resulting in high-purity lignins with no signals for cellulose, hemicellulose, or other impurities in two-dimensional heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectra. Different biomass particle sizes used for the organosolv pulping (1.6-2.0 mm (1); 0.5-1.0 mm (2); <0.25 mm (3)) influenced the molecular weight and chemical structure of the isolated lignins. Principal component analysis (PCA) of H NMR data revealed a high intergroup variance of and lignins, separating the small particle fraction from the larger ones. Furthermore, monolignol ratios identified via HSQC NMR differ significantly: lignins were composed of all three monolignols (guaiacyl (G), -hydroxyphenyl (H), syringyl (S)), while for and lignins only G and S units were observed (except for P3).
采用无催化剂的有机溶剂法制浆工艺对黄麻（，S）、芒草（，M）和泡桐树（，P）进行处理，得到二维异核单量子相干（HSQC）核磁共振（NMR）谱中无纤维素、半纤维素或其他杂质信号的高纯度木质素。用于有机溶剂法制浆的不同生物质颗粒大小（1.6-2.0 毫米（1）；0.5-1.0 毫米（2）；<0.25 毫米（3））影响了分离木质素的分子量和化学结构。H NMR 数据的主成分分析（PCA）显示出和 木质素之间的高组间方差，将小颗粒部分与较大颗粒部分分开。此外，通过 HSQC NMR 鉴定的愈创木基醇比例差异显著：木质素由三种愈创木基醇（愈创木基（G）、对羟基苯基（H）、丁香基（S））组成，而 木质素和 木质素仅观察到 G 和 S 单元（P3 除外）。
