Department of Internal Medicine I, Goethe University Frankfurt, 60590 Frankfurt, Germany.
Institute of Molecular Medicine and Experimental Immunology, University Clinic, 53127 Bonn, Germany.
Int J Mol Sci. 2020 Mar 16;21(6):2027. doi: 10.3390/ijms21062027.
Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.
近年来，用于研究肝脏疾病的分子和细胞研究方法有了极大的改进。先进的技术为建立具有优异纯度的细胞分离技术提供了新的机会，为 2D 和 3D 显微镜和高通量分析（例如批量或单细胞 RNA 测序）铺平了道路。干细胞和类器官研究的应用将有助于解析肝脏疾病的病理生理学以及各种实质细胞和非实质细胞之间的相互作用。此外，复杂的肝脏疾病动物模型允许在体内评估肝纤维化、门静脉高压和肝细胞癌（HCC），并对治疗策略进行临床前测试。本文的目的是详细描述在 2018 年 10 月于德国波恩举行的第八届欧洲肝实质细胞生物学俱乐部（ECLCB-8）会议的研讨会上提出的用于研究肝实质细胞生物学的新型体外和体内方法。
