Fan Wentao, Huang Yuwei, Wang Lisheng, Liao Weiguo, Li Zhou, Wu Yinai, Liao Fengyun, Yu Jianye, Liu Qiang
Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Department of Pharmacy, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
J Tradit Chin Med. 2017 Aug;37(4):503-509.
To investigate the effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.
Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu (BL 13) acupoint group, the Dazhui (GV 14) acupoint group, and the nonacupoint group or control group. Using microdialysis technology, preprocessed skin probes were implanted into the rats at Feishu (BL 13), Dazhui (GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu (BL 13), Dazhui (GV 14) acupoints, and the nonacupoint for all groups. Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.
The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics (r > 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.
Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood.
探讨针刺肺俞穴（BL 13）和大椎穴（GV 14）对用于哮喘治疗的凝胶剂中芥子碱硫氰酸盐透皮吸收的影响。
采用随机数字表法将30只Sprague-Dawley大鼠随机分为3组，每组10只：肺俞穴（BL 13）组、大椎穴（GV 14）组和非穴位组（对照组）。运用微透析技术，将预处理后的皮肤探针分别植入大鼠的肺俞穴（BL 13）、大椎穴（GV 14）和非穴位部位。然后，在所有组的肺俞穴（BL 13）、大椎穴（GV 14）和非穴位处皮肤涂抹哮喘凝胶。每隔30分钟收集一次透析液，共收集12小时。采用高效液相色谱法测定皮肤中芥子碱硫氰酸盐的归一化浓度。
大鼠体内透皮实验表明，量-时方程与零级动力学呈良好的线性关系（r>0.99）。透皮行为符合一级速率开放模型，穴位部位皮肤中芥子碱硫氰酸盐的透皮渗透率和累积量均高于非穴位部位皮肤。穴位组中芥子碱硫氰酸盐的全身最大浓度和曲线下面积显著高于非穴位组。两个穴位组均观察到有滞后时间，而非穴位组未观察到。
针刺穴位可促进芥子碱硫氰酸盐的经皮吸收，并控制其释放，减少血液中浓度波动。
