Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia.
Department of Biology, Faculty of Sciences, Taif University, Saudi Arabia.
Curr Pharm Biotechnol. 2020;21(14):1528-1538. doi: 10.2174/1389201021666200318114817.
In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments are effective, breast cancer cells develop resistance to these treatments. Numerous studies have demonstrated that active compounds in plant extracts, such as the phenolic compound Rosmarinic Acid (RA), exert anti-cancer effects.
We investigated the anticancer properties of methanolic crude extracts of seedlings and calli of Rosmarinus officinalis and Coleus hybridus, two Lamiaceae species.
MCF-7 human breast cancer cells were treated with methanolic crude extracts obtained from plant calli and seedlings generated in vitro, and cell proliferation was evaluated. Transcriptional profiling of the seedling and callus tissues was also conducted.
The mRNA expression levels of RA genes were higher in C. hybridus seedlings than in R. officinalis seedlings, as well as in C. hybridus calli than in R. officinalis calli, except for TAT and C4H. In addition, seedling and callus extracts of both R. officinalis and C. hybridus showed anti-proliferative effects against MCF-7 cells after 24 or 48 h of treatment.
At a low concentration of 10 μg/mL, C. hybridus calli and seedling extracts showed the most significant anti-proliferative effects after 24 and 48 h of exposure (p < 0.01); controls (doxorubicin) also showed significant inhibition, but lesser than that observed with C. hybridus (p < 0.05). Results with R. officinalis callus and seedling extracts did not significantly differ from those with untreated cells.
Methanolic extracts of R. officinalis and C. hybridus are potentially valuable options for breast cancer treatment.
在沙特阿拉伯，乳腺癌的发病率和死亡率都很高。尽管目前的治疗方法有效，但乳腺癌细胞会对这些治疗产生耐药性。许多研究表明，植物提取物中的活性化合物，如酚类化合物迷迭香酸（RA），具有抗癌作用。
我们研究了唇形科迷迭香和彩叶草幼苗和愈伤组织的甲醇粗提取物的抗癌特性。
用从植物愈伤组织和体外培养的幼苗中获得的甲醇粗提取物处理 MCF-7 人乳腺癌细胞，并评估细胞增殖情况。还对幼苗和愈伤组织的转录谱进行了分析。
与迷迭香幼苗相比，彩叶草幼苗的 RA 基因 mRNA 表达水平更高，与迷迭香愈伤组织相比，彩叶草愈伤组织的 RA 基因 mRNA 表达水平也更高，TAT 和 C4H 基因除外。此外，迷迭香和彩叶草的幼苗和愈伤组织提取物在处理 MCF-7 细胞 24 或 48 小时后均表现出抗增殖作用。
在低浓度 10μg/ml 时，彩叶草愈伤组织和幼苗提取物在暴露 24 和 48 小时后表现出最显著的抗增殖作用（p<0.01）；对照物（阿霉素）也表现出显著的抑制作用，但低于彩叶草（p<0.05）。迷迭香愈伤组织和幼苗提取物的结果与未处理的细胞没有显著差异。
迷迭香和彩叶草的甲醇提取物可能是治疗乳腺癌的有价值选择。
