Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey.
Plant Foods Hum Nutr. 2010 Jun;65(2):158-63. doi: 10.1007/s11130-010-0166-4.
The leaves of Rosmarinus officinalis harvested from three different locations of Turkey were extracted by both methanolic and supercritical CO(2) extraction. Subsequently, six extracts and the active compounds, carnosic acid, and rosmarinic acid were applied to various human cancer cell lines including NCI-H82 (human, small cell lung, carcinoma), DU-145 (human, prostate, carcinoma), Hep-3B (human, black, liver, carcinoma, hepatocellular), K-562 (human chronic myeloid leukemia), MCF-7 (human, breast, adenocarcinoma), PC-3 (human, prostate, adenocarcinoma) and MDA-MB-231 (human, breast, adenocarcinoma) by MTT assay. Supercritical CO(2) extracts had superior antiproliferative effect compared to the soxhlet extracts. Although the extracts exhibited various cytotoxic effects against different cell lines, comparatively low IC(50) values ranging between 12.50 and 47.55 microg/ml were attained against K-562, being the most sensitive cell line. Moreover, carnosic acid caused the lowest cell viability with values ranging from 13 to 30 % at a concentration of 19 muM after 48 h of treatments, resulting in superior antiproliferative effect. Rosemary extract is a potential candidate to be included in the anti-cancer diet with pre-determined doses avoiding toxicity.
从土耳其三个不同地点收获的迷迭香叶通过甲醇和超临界 CO(2)提取。随后，将六种提取物和活性化合物，迷迭香酸和迷迭香酸应用于各种人类癌细胞系，包括 NCI-H82（人，小细胞肺癌，癌），DU-145（人，前列腺，癌），Hep-3B（人，黑肝，癌，肝细胞），K-562（人慢性髓细胞白血病），MCF-7（人，乳腺，腺癌），PC-3（人，前列腺，腺癌）和 MDA-MB-231（人，乳腺，腺癌）通过 MTT 测定。与索氏提取相比，超临界 CO(2)提取物具有更好的抗增殖作用。尽管提取物对不同细胞系表现出不同的细胞毒性作用，但在针对 K-562（最敏感的细胞系）的测定中，获得了介于 12.50 和 47.55 μg/ml 之间的相对较低的 IC(50)值。此外，在 48 小时的处理后，迷迭香酸在 19 μM 的浓度下导致最低的细胞活力，范围为 13 至 30%，从而产生了更好的抗增殖作用。迷迭香提取物是一种潜在的候选物，可以包含在抗癌饮食中，以避免毒性。
