Klein-Junior Celso-Afonso, Zimmer Roberto, Borghetti Diana-Lina-Bronca, Portella Fernando-Freitas, Abich Flávia-Carolina, Marinowic Daniel-Rodrigo, Hosaka Keiichi, Reston Eduardo-Galia
Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil (ULBRA), Cachoeira do Sul, Rio Grande do Sul, Brazil.
Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil.
J Clin Exp Dent. 2020 Mar 1;12(3):e215-e219. doi: 10.4317/jced.56590. eCollection 2020 Mar.
The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers can cause pulp cell damage. The aim of this study was to evaluate the influence of a warm and hot air stream on the cytotoxicity of light-cured calcium hydroxide based cements.
The materials Dycal (conventional cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) were submitted to cytotoxicity analysis after polymerization, without vs. with previous heat treatment with a warm (37°C) and a hot (60°C) air stream. Following polymerization, cements were maintained in culture medium for 24 hours and 7 days, and subjected to the MTT test. Data were analyzed using analysis of variance (ANOVA) followed by post-hoc Student-Newman-Keuls (<0.05).
The results indicated significant differences between the materials according to their composition, i.e., light-cured cements treated with a jet of warm air showed similar cytotoxicity levels to those observed for conventional cement, suggesting that they may be considered alternatives in cases requiring pulp-capping treatment.
Application of a hot air stream reduced cytotoxicity of materials tested. Dental pulp capping, dental cements, calcium hydroxide, cell survival.
光固化氢氧化钙基水门汀存在聚合不完全的情况，未转化的单体可导致牙髓细胞损伤。本研究旨在评估温热气流和热气流对光固化氢氧化钙基水门汀细胞毒性的影响。
材料Dycal（传统水门汀）、Biocal、Hidrox-Cal和Ultra-Blend Plus（光固化氢氧化钙水门汀）在聚合后进行细胞毒性分析，分为未经温热（37°C）和热（60°C）气流预处理与经预处理两组。聚合后，水门汀在培养基中保存24小时和7天，并进行MTT试验。数据采用方差分析（ANOVA），随后进行事后Student-Newman-Keuls检验（<0.05）。
结果表明，根据材料组成，材料之间存在显著差异，即经温热气流喷射处理的光固化水门汀显示出与传统水门汀相似的细胞毒性水平，这表明在需要进行盖髓治疗的情况下，它们可被视为替代材料。
热气流的应用降低了受试材料的细胞毒性。牙髓盖髓术、牙科水门汀、氢氧化钙、细胞存活。
