HDAC3 在 db/db 小鼠 RGCs 中的动态表达及其与细胞凋亡和自噬的关系。

Dynamic Expression of HDAC3 in db/db Mouse RGCs and Its Relationship with Apoptosis and Autophagy.

机构信息

Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

出版信息

J Diabetes Res. 2020 Mar 1;2020:6086780. doi: 10.1155/2020/6086780. eCollection 2020.

BACKGROUND

Diabetic retinopathy (DR) is a severe complication of diabetes mellitus. DR is considered as a neurovascular disease. Retinal ganglion cell (RGC) loss plays an important role in the vision function disorder of diabetic patients. Histone deacetylase3 (HDAC3) is closely related to injury repair and nerve regeneration. The correlation between HDAC3 and retinal ganglion cells in diabetic retinopathy is still unclear yet.

METHODS

To investigate the chronological sequence of the abnormalities of retinal ganglion cells in diabetic retinopathy, we choose 15 male db/db mice (aged 8 weeks, 12 weeks, 16 weeks, 18 weeks, and 25 weeks; each group had 3 mice) as diabetic groups and 3 male db/m mice (aged 8 weeks) as the control group. In this study, we examined the morphological and immunohistochemical changes of HDAC3, Caspase3, and LC3B in a sequential manner by characterizing the process of retinal ganglion cell variation.

RESULTS

Blood glucose levels and body weights of db/db mice were significantly higher than that of the control group, < 0.01. Compared with the control group, the number of retinal ganglion cells decreased with the duration of disease increasing. HDAC3 expression gradually increased in RGCs of db/db mice. Caspase3 expression gradually accelerated in RGCs of db/db mice. LC3B expression dynamically changed in RGCs of db/db mice. HDAC3 was positively correlated with Caspase3 expression ( = 0.7424), < 0.01. Compared with the control group, the number of retinal ganglion cells decreased with the duration of disease increasing. HDAC3 expression gradually increased in RGCs of db/db mice. Caspase3 expression gradually accelerated in RGCs of db/db mice. LC3B expression dynamically changed in RGCs of db/db mice. HDAC3 was positively correlated with Caspase3 expression ( = 0.7424), < 0.01. Compared with the control group, the number of retinal ganglion cells decreased with the duration of disease increasing. HDAC3 expression gradually increased in RGCs of db/db mice. Caspase3 expression gradually accelerated in RGCs of db/db mice. LC3B expression dynamically changed in RGCs of db/db mice. HDAC3 was positively correlated with Caspase3 expression (. We clarified the dynamic expression changes of HDAC3, Caspase3, and LC3B in retinal ganglion cells of db/db mice. Our results suggest the HDAC3 expression has a positive correlation with apoptosis and autophagy.

背景

糖尿病视网膜病变（DR）是糖尿病的一种严重并发症。DR 被认为是一种神经血管疾病。视网膜神经节细胞（RGC）的损失在糖尿病患者的视力功能障碍中起着重要作用。组蛋白去乙酰化酶 3（HDAC3）与损伤修复和神经再生密切相关。HDAC3 与糖尿病视网膜病变中视网膜神经节细胞之间的相关性尚不清楚。

方法

为了研究糖尿病视网膜病变中视网膜神经节细胞异常的时间顺序，我们选择了 15 只雄性 db/db 小鼠（8 周、12 周、16 周、18 周和 25 周龄；每组 3 只）作为糖尿病组和 3 只雄性 db/m 小鼠（8 周龄）作为对照组。在这项研究中，我们通过对视网膜神经节细胞变化过程的特征描述，依次检查了 HDAC3、Caspase3 和 LC3B 的形态学和免疫组织化学变化。

结果

db/db 小鼠的血糖水平和体重明显高于对照组， < 0.01。与对照组相比，随着疾病持续时间的增加，视网膜神经节细胞的数量逐渐减少。HDAC3 在 db/db 小鼠的 RGC 中表达逐渐增加。Caspase3 在 db/db 小鼠的 RGC 中表达逐渐加快。LC3B 在 db/db 小鼠的 RGC 中动态变化。HDAC3 与 Caspase3 的表达呈正相关（ = 0.7424）， < 0.01。与对照组相比，随着疾病持续时间的增加，视网膜神经节细胞的数量逐渐减少。HDAC3 在 db/db 小鼠的 RGC 中表达逐渐增加。Caspase3 在 db/db 小鼠的 RGC 中表达逐渐加快。LC3B 在 db/db 小鼠的 RGC 中动态变化。HDAC3 与 Caspase3 的表达呈正相关（ = 0.7424）， < 0.01。与对照组相比，随着疾病持续时间的增加，视网膜神经节细胞的数量逐渐减少。HDAC3 在 db/db 小鼠的 RGC 中表达逐渐增加。Caspase3 在 db/db 小鼠的 RGC 中表达逐渐加快。LC3B 在 db/db 小鼠的 RGC 中动态变化。HDAC3 与 Caspase3 的表达呈正相关（ = 0.7424）， < 0.01。与对照组相比，随着疾病持续时间的增加，视网膜神经节细胞的数量逐渐减少。HDAC3 在 db/db 小鼠的 RGC 中表达逐渐增加。Caspase3 在 db/db 小鼠的 RGC 中表达逐渐加快。LC3B 在 db/db 小鼠的 RGC 中动态变化。HDAC3 与 Caspase3 的表达呈正相关（ = 0.7424）， < 0.01。