Department of Hand Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, P.R. China.
Department of Plastic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou City, P.R. China.
Cancer Biother Radiopharm. 2020 May;35(4):307-312. doi: 10.1089/cbr.2019.3149. Epub 2020 Mar 19.
Long noncoding RNA (lncRNA) LUADT1 is a known oncogenic lncRNA in lung cancer. This study aimed to explore the roles of LUADT1 in melanoma. Sixty pairs of melanoma and nontumor tissues were obtained from 60 melanoma patients (37 men and 23 women, 38-68 years, 52.1 ± 4.9 years) at the First Affiliated Hospital of Zhejiang University School of Medicine. Gene expression was analyzed by quantitative polymerase chain reaction and western blot. Cell transfections were performed to analyze gene expression. We found that LUADT1 was upregulated in melanoma and high levels of LUADT1 predicted poor survival. RNA interaction prediction showed that LUADT1 can form base pairing with miR-28-5p. In melanoma cells, LUADT1 overexpression mediated the upregulated Ras-related protein Rap-1b (RAP1B). Cell proliferation assay showed that LUADT1 and RAP1B overexpression mediated the increased proliferation rate of melanoma cells. In addition, miR-28-5p overexpression played opposite roles attenuating the effects of LUADT1 overexpression on both RAP1B expression and cancer cell proliferation. LUADT1 in melanoma and may sponge miR-28-5p to upregulate RAP1B, thereby promoting cancer cell proliferation.
长链非编码 RNA（lncRNA）LUADT1 是肺癌中一种已知的致癌 lncRNA。本研究旨在探讨 LUADT1 在黑色素瘤中的作用。 从浙江大学医学院第一附属医院 60 名黑色素瘤患者（37 名男性和 23 名女性，38-68 岁，52.1±4.9 岁）中获得了 60 对黑色素瘤和非肿瘤组织。通过定量聚合酶链反应和 Western blot 分析基因表达。通过细胞转染分析基因表达。 我们发现 LUADT1 在黑色素瘤中上调，高水平的 LUADT1 预示着预后不良。RNA 相互作用预测表明，LUADT1 可以与 miR-28-5p 形成碱基配对。在黑色素瘤细胞中，LUADT1 过表达介导 Ras 相关蛋白 Rap-1b（RAP1B）的上调。细胞增殖试验表明，LUADT1 和 RAP1B 过表达介导黑色素瘤细胞增殖率的增加。此外，miR-28-5p 过表达发挥相反的作用，减弱了 LUADT1 过表达对 RAP1B 表达和癌细胞增殖的影响。 LUADT1 在黑色素瘤中可能通过海绵吸附 miR-28-5p 而上调 RAP1B，从而促进癌细胞增殖。
