Peng Guotao, He Yuan, Wang Xiaoxiao, Cheng Yan, Zhang Haiyuan, Savolainen Kai, Mädler Lutz, Pokhrel Suman, Lin Sijie
College of Environmental Science and Engineering, Biomedical Multidisciplinary Innovation Research Institute, Shanghai East Hospital, Tongji University, Shanghai 200092, China.
Key Laboratory of Yangtze River Water Environment, Ministry of Education, Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
ACS Nano. 2020 Apr 28;14(4):4166-4177. doi: 10.1021/acsnano.9b08938. Epub 2020 Mar 24.
Redox-active metal oxide nanoparticles show varying oxidizing capacities and injury potentials toward biological systems. Here, two metal oxide libraries including transition-metal-doped CoO and PdO-CoO with strong chemical contacts were design-synthesized and used to investigate their biological injury potential and mechanisms using zebrafish as a model organism. Among different dopants, Cu significantly increased the oxidizing capacity of CoO. An increased amount of PdO resulted in higher density of heterojunctions, which also led to higher oxidizing capacity. The oxidizing capacity of these nanoparticles was positively correlated with higher mortality of dechorionated embryos and severe larval skin injury upon exposure. Using transgenic zebrafish (:eGFP), we show in real time that the redox-active nanoparticles induced skin injury and activated the infiltration of immune cells. Such inflammatory response was confirmed by the increased mRNA expression level of , , and genes. Although the exposure to the nanoparticles alone was not lethal, the skin injury did lower the tolerance level against other environmental contaminants. More importantly, after withdrawing from the nanoparticle exposure, larvae with skin injury could recover within 24 h in uncontaminated medium, indicating such injury was transient and recoverable.
氧化还原活性金属氧化物纳米颗粒对生物系统表现出不同的氧化能力和损伤潜力。在此，设计合成了两个包含具有强化学接触的过渡金属掺杂CoO和PdO-CoO的金属氧化物库，并以斑马鱼作为模式生物来研究它们的生物损伤潜力及机制。在不同的掺杂剂中，Cu显著提高了CoO的氧化能力。PdO含量的增加导致异质结密度更高，这也导致了更高的氧化能力。这些纳米颗粒的氧化能力与去壳胚胎的较高死亡率以及暴露后幼虫严重的皮肤损伤呈正相关。利用转基因斑马鱼（:eGFP），我们实时显示氧化还原活性纳米颗粒诱导皮肤损伤并激活免疫细胞浸润。 、 和 基因的mRNA表达水平升高证实了这种炎症反应。虽然单独暴露于纳米颗粒并不致命，但皮肤损伤确实降低了对其他环境污染物的耐受水平。更重要的是，在停止纳米颗粒暴露后，有皮肤损伤的幼虫在未受污染的培养基中可在24小时内恢复，表明这种损伤是短暂且可恢复的。
