化学定义培养基支持人类间充质干细胞的扩增。
Chemical-defined medium supporting the expansion of human mesenchymal stem cells.
机构信息
Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Department of Immunology, School of Medicine, Shenzhen University, Nanhai Avenue 3688, Shenzhen, 518060, Guangdong, People's Republic of China.
Department of Obstetrics, People's Hospital of Baoan, Shenzhen, 518055, People's Republic of China.
出版信息
Stem Cell Res Ther. 2020 Mar 19;11(1):125. doi: 10.1186/s13287-020-01641-7.
OBJECTIVES
Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet.
MATERIALS AND METHODS
Here, we developed the full chemical-defined medium, NBVbe medium, via RNA sequencing, bioinformatic analysis, and growth factor screening.
RESULTS
The NBVbe medium contains N2B27 medium with the BSA (bovine serum albumin) replaced by the recombinant human albumin, bFGF (basic fibroblast growth factor), vitamin C, and EGF (epidermal growth factor). The NBVbe medium could support the isolation and expansion of human MSCs from the umbilical cords.
CONCLUSIONS
The full chemical-defined medium supporting the isolation and expansion of human MSCs has been developed. This would be helpful for further optimization of the MSC medium, their clinical applications, and molecular characterization.
目的
间充质干细胞（MSCs）的治疗潜力已得到深入研究。然而，完全化学成分定义的培养基来支持人 MSCs 的分离和扩增尚未开发出来。
材料与方法
在此，我们通过 RNA 测序、生物信息学分析和生长因子筛选开发了完全化学成分定义的培养基 NBVbe 培养基。
结果
NBVbe 培养基包含 N2B27 培养基，其中 BSA（牛血清白蛋白）被重组人白蛋白、bFGF（碱性成纤维细胞生长因子）、维生素 C 和 EGF（表皮生长因子）取代。NBVbe 培养基可支持从脐带中分离和扩增人 MSCs。
结论
已开发出支持人 MSCs 分离和扩增的完全化学成分定义的培养基。这将有助于进一步优化 MSC 培养基、其临床应用和分子特征。
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