Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Coopers Plains, QLD 4108, Australia.
Food Chem. 2020 Jul 30;319:126515. doi: 10.1016/j.foodchem.2020.126515. Epub 2020 Feb 28.
An ultra-high-performance liquid chromatography-diode array detector-mass spectrometry method was developed for characterisation and quantification of anthocyanin components in complex corn-kernel matrices. The anthocyanin profiles and total anthocyanin content (TAC) of mature seeds of five types of anthocyanin-pigmented corn were reported. Internal standard was used to validate the efficiency of extraction and optimise the liquid extraction procedure for anthocyanins. A total of eighteen anthocyanins were identified and quantified. Cyanidin-based glucosides were the major pigments of purple-pericarp sweetcorn (75.5% of TAC) and blue-aleurone maize (91.6%), while pelargonidin-based glucosides composed the main anthocyanins of reddish-purple-pericarp sweetcorn (61.1%) and cherry-aleurone maize (74.6%). Importantly, previous studies reported the presence of acetylated and succinylated anthocyanins in corn kernels; these compounds were found to be artefact pigments, generated during the extraction process. These crucial findings provide the correct anthocyanin profiles of pigmented corns, and emphasise the importance of using acidified solutions for the extraction of corn-based anthocyanins.
建立了一种超高效液相色谱-二极管阵列检测-质谱联用方法，用于鉴定和定量复杂玉米籽粒基质中的花色苷成分。报告了五种花色苷着色玉米成熟种子的花色苷图谱和总花色苷含量（TAC）。内标用于验证提取效率并优化花色苷的液提取程序。共鉴定和定量了十八种花色苷。玉米中花青素的主要色素为矢车菊素葡萄糖苷（紫色果皮甜玉米 TAC 的 75.5%）和飞燕草素葡萄糖苷（蓝色糊粉层玉米 91.6%），而矢车菊素葡萄糖苷则是红紫色果皮甜玉米（61.1%）和樱桃糊粉层玉米（74.6%）的主要花色苷。重要的是，先前的研究报道了玉米籽粒中存在乙酰化和琥珀酰化的花色苷；这些化合物被认为是在提取过程中产生的人工色素。这些关键发现提供了着色玉米的正确花色苷图谱，并强调了在提取玉米基花色苷时使用酸化溶液的重要性。
