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帕氏果实中花色苷的亚临界水萃取、超高效液相色谱-三重四极杆飞行时间串联质谱分析、抗氧化及α-葡萄糖苷酶抑制活性的优化

Optimization of subcritical water extraction, UPLC-triple-TOF-MS/MS analysis, antioxidant and α-glucosidase inhibitory activities of anthocyanins from Pall. fruits.

作者信息

Ren Lichengcheng, Dong Qi, Liu Zhenhua, Wang Yue, Tan Nixia, Wang Honglun, Hu Na

机构信息

Qinghai Provincial Key Laboratory of Tibetan Medicine Research and CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, 810008, Xining, PR China.

School of Medicine, Qinghai University, Xining, Qinghai 810001, China.

出版信息

Food Chem X. 2024 Jul 6;23:101626. doi: 10.1016/j.fochx.2024.101626. eCollection 2024 Oct 30.

DOI:10.1016/j.fochx.2024.101626
PMID:39108629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11301309/
Abstract

This study aimed to optimize subcritical water extraction process, characterize chemical composition and investigate the biological activities of Pall. (NSP) anthocyanin. Overall, the optimization was achieved under following conditions: extraction temperature 140 °C, extraction time 45 min and flow rate 7 mL/min with the extraction yield of 1.075 mg/g. 3 cyanidin, 3 petunidin, 1 delphinidin and 1 pelargonidin compounds were identified in the anthocyanic extract from NSP via UPLC-Triple-TOF-MS/MS. NSP anthocyanin exhibited better DPPH free-radical scavenging activity than ascorbic acid. It displayed superior α-glucosidase inhibition activity, which was ∼14 times higher than that of acarbose. Moreover, enzyme kinetics results indicated that NSP anthocyanin behaved as a reversible, mixed-type inhibitor. Molecular docking and molecular dynamics simulation results revealed that NSP anthocyanin interacted with α-glucosidase mainly via van der Waals forces, hydrogen bond and possessed fairly stable configuration. Therefore, NSP anthocyanin is a promising α-glucosidase inhibitor for diabetes mellitus.

摘要

本研究旨在优化亚临界水提取工艺，表征化学组成，并研究紫露草（NSP）花青素的生物活性。总体而言，在以下条件下实现了优化：提取温度140℃，提取时间45分钟，流速7毫升/分钟，提取产率为1.075毫克/克。通过超高效液相色谱-三重四极杆飞行时间串联质谱（UPLC-Triple-TOF-MS/MS）在NSP的花色苷提取物中鉴定出3种矢车菊素、3种矮牵牛素、1种飞燕草素和1种天竺葵素化合物。NSP花青素表现出比抗坏血酸更好的1,1-二苯基-2-三硝基苯肼（DPPH）自由基清除活性。它显示出优异的α-葡萄糖苷酶抑制活性，比阿卡波糖高约14倍。此外，酶动力学结果表明，NSP花青素表现为可逆的混合型抑制剂。分子对接和分子动力学模拟结果表明，NSP花青素与α-葡萄糖苷酶主要通过范德华力、氢键相互作用，且具有相当稳定的构象。因此，NSP花青素是一种有前途的用于治疗糖尿病的α-葡萄糖苷酶抑制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/9f3cba71faaf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/94dcf5fb7063/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/52f744e64b11/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/732e52022d7a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/787870a1c7c6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/9f3cba71faaf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/94dcf5fb7063/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/52f744e64b11/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/732e52022d7a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/787870a1c7c6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/459c/11301309/9f3cba71faaf/gr4.jpg

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