Nurkhasanah Annisa, Fardad Titouan, Carrera Ceferino, Setyaningsih Widiastuti, Palma Miguel
Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jalan Flora, Bulaksumur, Depok, Sleman, Yogyakarta 55281, Indonesia.
Department of Physical Measurements, Institute of Technology of Lannion, CEDEX, 22302 Lannion, France.
Methods Protoc. 2023 Jul 30;6(4):69. doi: 10.3390/mps6040069.
This study aimed to determine the optimal UAE conditions for extracting anthocyanins from pigmented corn using the Box-Behnken design (BBD). Six anthocyanins were identified in the samples and were used as response variables to evaluate the effects of the following working variables: extraction solvent pH (2-7), temperature (10-70 °C), solvent composition (0-50% methanol in water), and ultrasound power (20-80%). The extraction time (5-25 min) was evaluated for complete recovery. Response surface methodology suggested optimal conditions, specifically 36% methanol in water with pH 7 at 70 °C using 73% ultrasound power for 10 min. The method was validated with a high level of accuracy (>90% of recovery) and high precision (CV < 5% for both repeatability and intermediate precision). Finally, the proposed analytical extraction method was successfully applied to determine anthocyanins that covered a wide concentration range (36.47-551.92 mg kg) in several pigmented corn samples revealing potential varieties providing more health benefits.
本研究旨在使用Box-Behnken设计（BBD）确定从有色玉米中提取花青素的最佳超声辅助提取（UAE）条件。在样品中鉴定出六种花青素，并将其用作响应变量，以评估以下工作变量的影响：提取溶剂pH值（2至7）、温度（10至70°C）、溶剂组成（水中甲醇含量为0至50%）和超声功率（20至80%）。对提取时间（5至25分钟）进行评估以实现完全回收。响应面法给出了最佳条件，具体为在70°C下，使用73%的超声功率处理10分钟，溶剂为pH值7的36%甲醇水溶液。该方法经验证具有高准确度（回收率>90%）和高精密度（重复性和中间精密度的变异系数均<5%）。最后，所提出的分析提取方法成功应用于测定多个有色玉米样品中浓度范围较宽（36.47至551.92毫克/千克）的花青素，揭示了具有更多健康益处的潜在品种。
