Microwave-assisted deep eutectic solvent extraction and macroporous resin purification of anthocyanins from : Process optimization, structural characterization and quantitative analysis.

A novel approach for extraction and purification of the anthocyanins from was developed by microwave-assisted deep eutectic solvent extraction (MAE-DES) coupled to macroporous resin separation (MRS). Citric acid-based DES as the extractant was screened and characterized from 13 synthesized deep eutectic solvents due to the excellent performance. Through RSM optimization of MAE-DES process, the anthocyanin yield reached 6.442 ± 0.135 mg/g, demonstrating that MAE-DES significantly superior to that of conventional methods. With AB-8 MRS purification, the anthocyanin purity was increased by 63-fold. By UHPLC-Q-TOF-MS/MS, the anthocyanins obtained from were dominantly composed of pelargonins. Based on composition analysis, pH differential method was first modified by introducing weighting factors, avoiding serious underestimation of the anthocyanins content in . MAE-DES and the modified spectrophotometry were successfully applied for assessment of anthocyanin extraction and sample drying methods, providing an efficient alternative to development and application of the anthocyanins from .