Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Beutenbergstraße 11a, 07745 Jena, Germany.
University of Potsdam, Institute for Biochemistry and Biology, Karl-Liebknecht Str. 24-25, 14476 Potsdam, Germany.
Org Lett. 2020 Apr 3;22(7):2634-2638. doi: 10.1021/acs.orglett.0c00601. Epub 2020 Mar 20.
Growth from spores activated a biosynthetic gene cluster in sp. RB29, resulting in the identification of two novel groups of halogenated polyketide natural products, named maduralactomycins and actinospirols. The unique tetracyclic and spirocyclic structures were assigned based on a combination of NMR analysis, chemoinformatic calculations, X-ray crystallography, and C labeling studies. On the basis of HRMS data, genome mining, and gene expression studies, we propose an underlying noncanonical angucycline biosynthesis and extensive post-polyketide synthase (PKS) oxidative modifications.
从孢子中生长出来的生物合成基因簇激活了 RB29 中的 sp.，从而鉴定出两种新型卤代聚酮天然产物，命名为 maduralactomycins 和 actinospirols。基于 NMR 分析、化学信息计算、X 射线晶体学和 C 标记研究的组合，确定了独特的四环和螺环结构。根据高分辨率质谱数据、基因组挖掘和基因表达研究，我们提出了一种潜在的非经典蒽环类生物合成和广泛的聚酮合酶（PKS）氧化修饰。
