Department of Urology Surgery, Lianshui County People's Hospital, Huaian, China.
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2264-2270. doi: 10.26355/eurrev_202003_20492.
The importance of circular RNAs in malignant tumors has attracted a lot of attention. Circular PSMC3 (CircPSMC3) is identified as a tumor suppressor in gastric cancer. The role of circPSMC3 in prostate cancer (PCa) remains unclear. Our study aims to uncover whether and how circPSMC3 functions in PCa development.
Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to determine the level of circPSMC3 in PCa tissues and cell lines. The relation between circPSMC3 expression and patients' prognosis was analyzed as well. CircPSMC3 lentivirus was constructed and transfected into PCa cells. Cell migration and invasion abilities were detected through wound healing assay, transwell assay, and Matrigel assay, respectively. Western blot assay was performed to detect the protein level of DGCR8.
CircPSMC3 was lowly expressed in PCa tissues compared with adjacent normal tissues. Low expression of circPSMC3 was significantly downregulated in PCa cell lines as well. The migration and invasion abilities of PCa cells were significantly inhibited after circPSMC3 was overexpressed in vitro. Furthermore, DGCR8 expression increased remarkably via the overexpression of circPSMC3.
CircPSMC3 could suppress PCa cell migration and invasion by upregulating DGCR8.
环状 RNA 在恶性肿瘤中的重要性引起了广泛关注。环状 PSMC3（CircPSMC3）被鉴定为胃癌中的肿瘤抑制因子。CircPSMC3 在前列腺癌（PCa）中的作用尚不清楚。我们的研究旨在揭示 CircPSMC3 是否以及如何在 PCa 发展中发挥作用。
实时定量聚合酶链反应（RT-qPCR）用于检测 PCa 组织和细胞系中 CircPSMC3 的水平。分析 CircPSMC3 表达与患者预后的关系。构建 CircPSMC3 慢病毒并转染至 PCa 细胞中。通过划痕愈合实验、Transwell 实验和 Matrigel 实验分别检测细胞迁移和侵袭能力。Western blot 实验检测 DGCR8 的蛋白水平。
与相邻正常组织相比，PCa 组织中 CircPSMC3 的表达水平较低。PCa 细胞系中 CircPSMC3 的表达水平也显著下调。体外过表达 CircPSMC3 后，PCa 细胞的迁移和侵袭能力显著受到抑制。此外，CircPSMC3 的过表达显著增加了 DGCR8 的表达。
CircPSMC3 通过上调 DGCR8 抑制 PCa 细胞的迁移和侵袭。
