Liu Jun, Ding Jinli, Qu Bing, Liu Jiuying, Song Xiaojie, Suo Qingli, Zhou Aifen, Yang Jing
Reproductive Medical Center, Renmin Hospital, Wuhan University, Wuhan, China.
Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science &Technology, Wuhan, China.
J Cell Mol Med. 2020 Sep;24(18):11001-11011. doi: 10.1111/jcmm.15747. Epub 2020 Aug 17.
Polycystic ovary syndrome (PCOS), the most common female endocrine disease that causes anovulatory infertility, still lacks promising strategy for the accurate diagnosis and effective therapeutics of PCOS attributed to its unclear aetiology. In this study, we determined the abnormal reduction in circPSMC3 expression by comparing the ovarian tissue samples of PCOS patients and normal individuals. The symptom relief caused by up-regulation of circPSMC3 in PCOS model mice suggested the potential for further study. In vitro functional experiments confirmed that circPSMC3 can inhibit cell proliferation and promote apoptosis by blocking the cell cycle in human-like granular tumour cell lines. Mechanism study revealed that circPSMC3 may play its role through sponging miR-296-3p to regulate PTEN expression. Collectively, we preliminarily characterized the role and possible insights of circPSMC3/miR-296-3p/PTEN axis in the proliferation and apoptosis of KGN cells. We hope that this work provides some original and valuable information for the research of circRNAs in PCOS, not only to better understand the pathogenesis but also to help provide new clues for seeking for the future therapeutic target of PCOS.
多囊卵巢综合征（PCOS）是导致无排卵性不孕的最常见的女性内分泌疾病，由于其病因不明，目前仍缺乏针对PCOS准确诊断和有效治疗的理想策略。在本研究中，通过比较PCOS患者和正常个体的卵巢组织样本，我们确定了circPSMC3表达异常降低。PCOS模型小鼠中circPSMC3上调引起的症状缓解提示了进一步研究的潜力。体外功能实验证实，circPSMC3可通过阻断人颗粒细胞瘤细胞系中的细胞周期来抑制细胞增殖并促进细胞凋亡。机制研究表明，circPSMC3可能通过吸附miR-296-3p来调节PTEN表达发挥其作用。我们初步阐明了circPSMC3/miR-296-3p/PTEN轴在KGN细胞增殖和凋亡中的作用及可能机制。我们希望这项工作为PCOS中circRNAs的研究提供一些原始且有价值的信息，不仅有助于更好地理解其发病机制，还能为寻找PCOS未来的治疗靶点提供新线索。
