环状 RNA_LARP4 通过靶向 FOXO3A 抑制前列腺癌细胞迁移和侵袭。
Circular RNA_LARP4 inhibits cell migration and invasion of prostate cancer by targeting FOXO3A.
机构信息
Department of Urology Surgery, Yinzhou People's Hospital, Yinzhou, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 May;24(10):5303-5309. doi: 10.26355/eurrev_202005_21312.
OBJECTIVE
The importance of circular RNAs in malignant tumors causes more attention in researchers. Circular RNA_LARP4 is identified as a tumor suppressor in gastric cancer, but the role of circular RNA_LARP4 in prostate cancer (PCa) remains unclear. Our work aims to uncover whether and how circular RNA_LARP4 functions in the PCa development.
PATIENTS AND METHODS
Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to determine the level of circular RNA_LARP4 in PCa tissues and cell lines. The patients' prognosis was analyzed. Circular RNA_LARP4 lentivirus was constructed for transfection of PCa cells. Cell migrated and invaded ability was detected through wound healing assay and transwell assay. Western blot assay was performed to analyze the protein level of FOXO3A.
RESULTS
The low circular RNA_LARP4 expression was associated with poor prognosis of PCa patients. The circular RNA_LARP4 was lowly expressed in PCa tissues compared with adjacent samples. The expression of circular RNA_LARP4 was downregulated in PCa cell lines. The cell migrated and invaded ability of PCa cells was inhibited after circular RNA_LARP4 was overexpressed. Furthermore, FOXO3A expression was increased via the overexpression of circular RNA_LARP4.
CONCLUSIONS
Circular RNA_LARP4 could suppress cell migration and invasion of PCa by upregulating FOXO3A.
目的
环状 RNA 在恶性肿瘤中的重要性引起了研究人员的更多关注。环状 RNA_LARP4 被确定为胃癌的肿瘤抑制因子，但环状 RNA_LARP4 在前列腺癌（PCa）中的作用尚不清楚。我们的工作旨在揭示环状 RNA_LARP4 是否以及如何在 PCa 发展中发挥作用。
患者和方法
实时定量聚合酶链反应（RT-qPCR）用于确定 PCa 组织和细胞系中环状 RNA_LARP4 的水平。分析患者的预后。构建环状 RNA_LARP4 慢病毒用于转染 PCa 细胞。通过划痕愈合实验和 Transwell 实验检测细胞迁移和侵袭能力。通过 Western blot 实验分析 FOXO3A 蛋白水平。
结果
低表达环状 RNA_LARP4 与 PCa 患者的不良预后相关。与相邻样本相比，PCa 组织中环状 RNA_LARP4 表达水平较低。PCa 细胞系中环状 RNA_LARP4 的表达下调。过表达环状 RNA_LARP4 后，PCa 细胞的迁移和侵袭能力受到抑制。此外，通过过表达环状 RNA_LARP4 ，FOXO3A 的表达增加。
结论
环状 RNA_LARP4 通过上调 FOXO3A 抑制 PCa 细胞的迁移和侵袭。
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