Technische Universität Darmstadt, Clemens-Schöpf Institute of Organic Chemistry and Biochemistry, Alarich-Weiss Str. 4, 64287, Darmstadt, Germany.
Technische Universität Darmstadt, Fachbereich Material- und Geowissenschaften, Fachgebiet Materialanalytik, Alarich-Weiss-Str. 2, 64287, Darmstadt, Germany.
Chemistry. 2020 Jul 14;26(39):8511-8517. doi: 10.1002/chem.202001160. Epub 2020 Jun 17.
A nanopore-based Cu -sensing system is reported that allows for an ultrasensitive and selective detection of Cu with the possibility for a broad range of applications, for example in medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His is employed to selectively bind Cu ions in the presence of Ni and Zn and was crafted into ion track-etched nanopores. Upon Cu binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu allowing to sense femtomolar Cu concentrations in human urine mimics. Washing with EDTA fully restores the Cu -binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu -sensing device, which will be of great importance for bedside diagnosis and monitor of Cu levels in patients with copper-dysfunctional homeostasis.
一种基于纳米孔的 Cu 传感系统被报道，可以实现对 Cu 的超灵敏和选择性检测，具有广泛的应用前景，例如在医学诊断中。采用荧光 ATCUN 样肽 5/6-FAM-Dap-β-Ala-His 选择性地结合 Cu 离子，在 Ni 和 Zn 存在的情况下，并将其制成离子刻蚀纳米孔。Cu 结合后，肽传感器的荧光被猝灭，从而可以检测溶液中的 Cu。肽修饰的纳米孔的离子传输特性对 Cu 具有极高的灵敏度和选择性，允许检测人尿模拟物中的飞摩尔 Cu 浓度。用 EDTA 洗涤可完全恢复传感器的 Cu 结合性能，实现多次重复测量。该系统的稳健性显然有可能进一步开发成一种易于使用的、芯片实验室的 Cu 传感装置，这对于床边诊断和监测铜功能失调的患者的 Cu 水平将非常重要。
