Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming, China.
Department of Stomatology, The First People's Hospital of Yunnan Provience, Kunming, China.
J Biomed Mater Res A. 2020 Aug 1;108(8):1713-1725. doi: 10.1002/jbm.a.36937. Epub 2020 Apr 15.
The extracellular matrix provides cells with a support structure and an attachment site in actual substrate. Its biochemical and surface properties play an important role in and have significant impact on cell attachment, proliferation, migration, differentiation, and gene expression. Leveraging the hydrophilicity and neuroprotective of gastrodin, a gastrodin/polyurethane (PU) elastomer was developed utilizing in situ polymerization and salt-leaching methods. The results showed that gastrodin/PU film had a good flexibility and supporting strength, as well as hydrophilicity. Thus film possessed highly surface area, interconnected porous structure with a pore size (10~60 μm) for cell attachment, and could provide surface cues to augment neurite extension. For PC12 cells cultured within the films, especially the 5gastrodin/PU group, presented a progressive increase with time, coupled with the upregulation of brain-derived neurotrophic factor and glial cell derived neurotrophic factor expression. This is the first report on the construction of a gastrodin/PU porous film, and the results reveal its promise as a scaffold material for neural tissue engineering.
细胞外基质为细胞提供了实际基质中的支撑结构和附着位点。其生化和表面特性在细胞附着、增殖、迁移、分化和基因表达中起着重要作用，并具有重大影响。利用天麻素的亲水性和神经保护作用，采用原位聚合和盐析法开发了一种天麻素/聚氨酯（PU）弹性体。结果表明，天麻素/PU 薄膜具有良好的柔韧性和支撑强度以及亲水性。因此，薄膜具有高表面积、相互连接的多孔结构，孔径为（10~60μm），有利于细胞附着，并能提供表面线索来促进神经突的延伸。对于在薄膜内培养的 PC12 细胞，特别是 5%天麻素/PU 组，随着时间的推移呈递增趋势，同时脑源性神经营养因子和胶质细胞源性神经营养因子的表达上调。这是关于构建天麻素/PU 多孔膜的首次报道，结果表明其有望成为神经组织工程的支架材料。
