School of Veterinary Medicine, University College Dublin (UCD), Dublin 4, Ireland.
Department of Agriculture, Food and the Marine, Dublin, Ireland.
Res Vet Sci. 2022 Dec;149:119-124. doi: 10.1016/j.rvsc.2022.06.011. Epub 2022 Jun 23.
Serological diagnosis of Mycobacterium bovis infection in badgers (Meles meles) has relied primarily on antibody recognition of MPB83, a sero-dominant antigen of M. bovis. Most vaccine studies in badgers to date have used the Bacille Calmette-Guerin (BCG) Danish strain, a low producer of MPB83. Due to a supply shortage of the BCG Danish strain, the BCG Sofia SL222 strain has been considered as an alternative vaccine. This strain is a high producer of MPB83 raising the possibility that vaccinated animals will test sero-positive in diagnostic assays that use this antigen. In this study we vaccinated a group of eleven badgers with BCG Sofia SL222 by injection via the intramuscular route and a booster vaccine dose was similarly delivered at 12 weeks and 64 weeks. Primary vaccination did not result in measured detection of antibodies against MPB83 in any badger during the first twelve weeks using serum or whole blood tested by the Dual Path Platform (DPP) VetTB, however, MPB83 antibodies were detected in a semi-quantitative ELISA assay. Following delivery of booster BCG at 12 weeks and 64 weeks, antibody responses against MPB83 were recorded in badgers using whole blood and serum on DPP VetTB and by ELISA. At all time points, vaccination was also associated with the in vitro production of gamma interferon (IFN-γ) following stimulation of lymphocytes with bovine and avian tuberculin (PPD) but not with MPB83 or M. bovis specific antigen CFP-10. The results indicate that serological diagnosis of tuberculosis using tests that target MPB83 may be compromised if badgers are repeatedly vaccinated with BCG Sofia.
牛型分枝杆菌感染的血清学诊断主要依赖于抗体对牛分枝杆菌血清优势抗原 MPB83 的识别。迄今为止，大多数针对獾的疫苗研究都使用了卡介苗（BCG）丹麦株，该株是 MPB83 的低生产者。由于 BCG 丹麦株供应短缺，BCG 索非亚 SL222 株已被视为替代疫苗。该菌株是 MPB83 的高生产者，这使得接种疫苗的动物在使用该抗原的诊断检测中可能会出现血清阳性。在这项研究中，我们通过肌肉内注射的方式给 11 只獾接种了 BCG 索非亚 SL222，在 12 周和 64 周时，同样给予了加强疫苗剂量。在最初的 12 周内，通过 Dual Path Platform (DPP) VetTB 用血清或全血检测，没有一只獾检测到针对 MPB83 的抗体，然而，在半定量 ELISA 检测中检测到了 MPB83 抗体。在 12 周和 64 周时给予加强 BCG 后，使用 DPP VetTB 和 ELISA 在全血和血清中记录了针对 MPB83 的抗体反应。在所有时间点，接种疫苗还与在体外用牛结核菌素（PPD）和禽结核菌素刺激淋巴细胞后产生伽马干扰素（IFN-γ）有关，但与 MPB83 或牛分枝杆菌特异性抗原 CFP-10 无关。结果表明，如果獾反复接种 BCG 索非亚，使用针对 MPB83 的检测进行结核病的血清学诊断可能会受到影响。
