Institute of Environment and Ecology, Shandong Normal University, Ji'nan, 250358, China.
Unit of Toxicology, Department of Zoology, Bharathiar University, Coimbatore, 641046, TamilNadu, India.
Chemosphere. 2020 Aug;252:126498. doi: 10.1016/j.chemosphere.2020.126498. Epub 2020 Mar 14.
Both Diheptyl-phthalate (DHpP) and Diisodecyl-phthalate (DIDP) were used extensively as plasticizers. Recently, their occurrence in the environmental matrices and human body fluids have been reported. Unfortunately, these phthalate congeners are without basic toxicity profiles. Hence, we studied the toxic effects of both DHpP and DIDP in the median lethal concentration (LC 96-h) on zebrafish (Danio rerio). We assessed swimming behavior strength and tissues biomarker responses including total antioxidants capacity (TAOC), transaminases, and acetylcholinesterase (AChE) enzyme. Fish exposed to phthalate congeners (Treatment-I and-II) for 15-days showed alterations on fish swimming behavior and circadian rhythm. At the end of the exposure period, both liver and heart tissue transaminases activities were found to be accelerated in DHpP and DIDP treated fish, when compared to control group. TAOC and AChE activities were found to be decreased in brain, gills, intestine, and muscle tissues of phthalate congeners treated fish than the control group. Alterations observed in the studied biomarkers were concentration-based response. Among treatment groups DHpP showed higher effects. Comparative studies on swimming behavior and biochemical activities were reasonable to know the swimming responses are mediated due to external stress or internal stress. More studies on molecular and biomarkers assessments are warranted on toxicity of emerging contaminants.
邻苯二甲酸二庚酯（DHpP）和邻苯二甲酸二异癸酯（DIDP）被广泛用作增塑剂。最近，它们在环境基质和人体体液中的存在已被报道。不幸的是，这些邻苯二甲酸同系物没有基本的毒性特征。因此，我们研究了 DHpP 和 DIDP 在斑马鱼（Danio rerio）中的半数致死浓度（LC96-h）对鱼类的毒性作用。我们评估了游泳行为强度和组织生物标志物反应，包括总抗氧化能力（TAOC）、转氨酶和乙酰胆碱酯酶（AChE）酶。暴露于邻苯二甲酸同系物（处理 I 和 II）15 天的鱼类表现出游泳行为和昼夜节律的改变。在暴露期结束时，与对照组相比，DHpP 和 DIDP 处理的鱼类肝脏和心脏组织中转氨酶的活性均加快。与对照组相比，在 DHpP 和 DIDP 处理的鱼类的脑组织、鳃组织、肠组织和肌肉组织中，TAOC 和 AChE 的活性均降低。研究生物标志物的变化是基于浓度的反应。在处理组中，DHpP 表现出更高的影响。对游泳行为和生化活性的比较研究可以合理地了解游泳反应是由外部应激还是内部应激介导的。需要对新兴污染物的毒性进行更多的分子和生物标志物评估研究。
