Sharma Deepika, Ledwani Lalita, Mehrotra Tarang, Kumar Naveen, Pervaiz Naveed, Kumar Ravinder
Manipal University Jaipur, Rajasthan 303007, India.
Manipal University Jaipur, Rajasthan 303007, India.
J Photochem Photobiol B. 2020 Feb 24;206:111841. doi: 10.1016/j.jphotobiol.2020.111841.
The synthesis of magnetic Hematite nanoparticles (α-FeO) via green route has been a long lasting challenge for the scientific and technological fascination of many researchers. In the present investigation, iron oxide nanoparticles (α-FeO) were synthesized using Rheum emodi roots in a cost effective and ecofriendly method. Their physicochemical property orchestration involved techniques such as UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray (EDX), X-ray diffraction (XRD), Thermogravimetric Analysis (TGA), Vibrating sample magnetometer (VSM), and Atomic force microscopy (AFM). Through TEM, FESEM and AFM analysis, α-FeONPs were confirmed spherical in shape and the average diameter of particle is ~12 nm as depicted through TEM image. Thermal property was investigated by TGA. Magnetic behavior was observed in R. emodi mediated α-FeONPs by magnetic hysteresis measurements. FTIR analysis revealed the presence of anthraquinones in R. emodi roots extract which play the central role in stabilization of the α-FeONPs. Further, the crystalline nature of the nanoparticle sample was determined with XRD experiment and SAED fringes calculation. The crystal was also confirmed with Rietveld refinement of XRD profile fitted with R-3c model Additionally, magnetic interaction with bacterial cell wall showed antimicrobial property against Escherichia coli, Gram-negative and Staphylococcus aureus, Gram-positive species. The approach transcribed in this paper reveals a novel methodology that utilizes α-FeO NPs to initiate apoptosis and inhibition of cervical cancer cells.
通过绿色路线合成磁性赤铁矿纳米颗粒（α-Fe₂O₃）长期以来一直是许多研究人员在科学技术方面感兴趣的挑战。在本研究中，使用大黄根茎以经济高效且环保的方法合成了氧化铁纳米颗粒（α-Fe₂O₃）。它们的物理化学性质调控涉及紫外可见光谱、傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、场发射扫描电子显微镜（FESEM）、能量色散X射线（EDX）、X射线衍射（XRD）、热重分析（TGA）、振动样品磁强计（VSM）和原子力显微镜（AFM）等技术。通过TEM、FESEM和AFM分析，证实α-Fe₂O₃纳米颗粒呈球形，如TEM图像所示，颗粒的平均直径约为12nm。通过TGA研究了热性能。通过磁滞测量在大黄介导的α-Fe₂O₃纳米颗粒中观察到了磁性行为。FTIR分析表明大黄根茎提取物中存在蒽醌，其在α-Fe₂O₃纳米颗粒的稳定中起核心作用。此外，通过XRD实验和SAED条纹计算确定了纳米颗粒样品的晶体性质。还用R-3c模型拟合的XRD图谱的Rietveld精修证实了晶体。此外，与细菌细胞壁的磁性相互作用显示出对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的抗菌特性。本文阐述的方法揭示了一种利用α-Fe₂O₃纳米颗粒引发宫颈癌细胞凋亡和抑制的新方法。
