炎性中耳疾病中高人类乳头瘤病毒DNA载量
High Human Papillomavirus DNA loads in Inflammatory Middle Ear Diseases.
作者信息
Malagutti Nicola, Rotondo John Charles, Cerritelli Luca, Melchiorri Claudio, De Mattei Monica, Selvatici Rita, Oton-Gonzalez Lucia, Stomeo Francesco, Mazzoli Manuela, Borin Michela, Mores Beatrice, Ciorba Andrea, Tognon Mauro, Pelucchi Stefano, Martini Fernanda
机构信息
ENT Department, University Hospital of Ferrara, 44121 Ferrara, Italy.
Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, Italy.
出版信息
Pathogens. 2020 Mar 18;9(3):224. doi: 10.3390/pathogens9030224.
. Previous studies reported human papillomaviruses (HPVs) in middle ear tumors, whereas these viruses have been poorly investigated in chronic inflammatory middle ear diseases. We investigated HPVs in non-tumor middle ear diseases, including chronic otitis media (COM). . COM specimens (n = 52), including chronic suppurative otitis media (CSOM) (n =38) and cholesteatoma (COMC) (n = 14), as well as normal middle ear (NME) specimens (n = 56) were analyzed. HPV sequences and DNA loads were analyzed by quantitative-PCR. HPV genotyping was performed by direct sequencing. . HPV DNA was detected in 23% (12/52) of COM and in 30.4% (17/56) of NME ( > 0.05). Specifically, HPV DNA sequences were found in 26.3% (10/38) of CSOM and in 14.3% (2/14) of COMC ( > 0.05). Interestingly, the HPV DNA load was higher in COMC (mean 7.47 copy/cell) than in CSOM (mean 1.02 copy/cell) and NME (mean 1.18 copy/cell) (P = 0.03 and P = 0.017 versus CSOM and NME, respectively). HPV16 and HPV18 were the main genotypes detected in COMC, CSOM and NME. . These data suggest that HPV may infect the middle ear mucosa, whereas HPV-positive COMCs are associated with higher viral DNA loads as compared to NME.
以往研究报道过人乳头瘤病毒（HPV）存在于中耳肿瘤中，而这些病毒在慢性炎症性中耳疾病中的研究较少。我们对包括慢性中耳炎（COM）在内的非肿瘤性中耳疾病中的HPV进行了研究。分析了52例COM标本，包括38例慢性化脓性中耳炎（CSOM）和14例胆脂瘤型中耳炎（COMC），以及56例正常中耳（NME）标本。通过定量PCR分析HPV序列和DNA载量。通过直接测序进行HPV基因分型。在23%（12/52）的COM和30.4%（17/56）的NME中检测到HPV DNA（P>0.05）。具体而言，在26.3%（10/38）的CSOM和14.3%（2/14）的COMC中发现了HPV DNA序列（P>0.05）。有趣的是，COMC中的HPV DNA载量（平均7.47拷贝/细胞）高于CSOM（平均1.02拷贝/细胞）和NME（平均1.18拷贝/细胞）（与CSOM和NME相比，P分别为0.03和0.017）。HPV16和HPV18是在COMC、CSOM和NME中检测到的主要基因型。这些数据表明HPV可能感染中耳黏膜，而与NME相比，HPV阳性的COMC与更高的病毒DNA载量相关。
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