Gaultier C, Reinberg A, Motohashi Y
Laboratoire de Physiologie, CNRS UA 1159 Hôpital A. Béclère, Clamart, France.
Chronobiol Int. 1988;5(3):285-90. doi: 10.3109/07420528809079566.
Six children (boys and girls, 8 to 13 years old) with allergic asthma (AA) had their total pulmonary resistance (R1) measured at four fixed times (0730, 1130, 1630 and 2230 hr), before and again 10 min after a 2 mg orciprenaline (beta-agonist) aerosol inhalation. R1 was measured by means of the esophageal balloon technique. Subjects were socially synchronized in May with a diurnal activity from 0700 to 2100 and a nocturnal rest. Patients had had no asthma attacks and had received no medication for 8-15 days. Time series were analyzed according to conventional (t-tested mean time point differences) ANOVA, and cosinor methods. The 24 hr adjusted means (cm H2O.l/s +/- SEM) were 5.7 +- 0.4 in seven previously documented healthy children. 7.4 +/- 1.2 in AA before orciprenaline, and 4.9 +/- 0.2 in healthy children, 5.2 +/- 0.8 in AA after beta-agonist inhalation. Circadian rhythms were detected in both groups before but not after treatment. The treatment had its maximal effect on R1 around 0730 (when bronchial patency is close to its trough) and had no effect around 1630 (peak time of airway patency) in both groups (P less than 0.01; ANOVA). Thus, inhaled orciprenaline was mainly effective around 0730 and to a lesser extent around 2230 whereas there was no detectable effect during the day (1230 and 1630).
六名患有过敏性哮喘（AA）的儿童（年龄在8至13岁之间，有男有女）在四个固定时间点（0730、1130、1630和2230时）测量了总肺阻力（R1），测量时间分别为吸入2毫克奥西那林（β-激动剂）气雾剂之前和之后10分钟。R1通过食管气囊技术进行测量。研究对象于五月进行了社交同步，白天活动时间为0700至2100，夜间休息。患者在8至15天内未发生哮喘发作且未接受任何药物治疗。时间序列根据传统的（t检验平均时间点差异）方差分析和余弦法进行分析。七名先前记录的健康儿童的24小时校正平均值（厘米水柱·升/秒±标准误）为5.7±0.4。过敏性哮喘患者在吸入奥西那林之前为7.4±1.2，健康儿童为4.9±0.2，吸入β-激动剂后过敏性哮喘患者为5.2±0.8。两组在治疗前均检测到昼夜节律，但治疗后未检测到。治疗对R1的最大作用出现在0730左右（此时支气管通畅度接近谷底），在两组中1630左右（气道通畅度的峰值时间）均无作用（P<0.01；方差分析）。因此，吸入奥西那林主要在0730左右有效，在2230左右效果较小，而在白天（1230和1630）未检测到明显效果。
