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基于蔗糖对新菌株sp. YI8的筛选及其在利用糖蜜生产聚羟基丁酸酯中的应用。

Sucrose-Based Screening of a Novel Strain, sp. YI8, and Its Application to Polyhydroxybutyrate Production from Molasses.

作者信息

Lee Yeda, Cho Dohyun, Shin Yuni, Han Yebin, Lim Gaeun, Jeon Jongmin, Yoon Jeongjun, Joo Jeongchan, Jeong Hwabong, Ahn Jungoh, Bhatia Shashi Kant, Yang Yunghun

机构信息

Advanced Materials Program, Department of Biological Engineering, College of Engineering, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.

Green & Sustainable Materials Research and Development Department, Korea Institute of Industrial Technology (KITECH), Cheonan 31056, Republic of Korea.

出版信息

Polymers (Basel). 2025 May 26;17(11):1471. doi: 10.3390/polym17111471.

DOI:10.3390/polym17111471
PMID:40508716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12158085/
Abstract

Poly(3-hydroxybutyrate) is a biodegradable plastic produced by various microbes. Considering the emerging environmental problems caused by plastics, P(3HB) has gained attention as a substitute for conventional plastics. In this study, we isolated a novel P(3HB)-producing microbe, sp. YI8, which utilized sucrose as a cost-effective carbon source for P(3HB) production. Under optimized conditions, sp. YI8 produced 6.2 g/L P(3HB) using sucrose as the sole carbon source. P(3HB) extracted from YI8 exhibited a pinkish color derived from a dye produced naturally by YI8. Films fabricated from extracted P(3HB) polymer were subjected to analyses, including gel permeation chromatography, universal test machine, and differential scanning calorimetry, to determine their physical properties. The obtained values were almost identical to those of P(3HB) films extracted from and H16. Overall, this study presents the potential of spp. YI8 as a P(3HB)-producing strain and the P(3HB) films extracted from this strain.

摘要

聚(3-羟基丁酸酯)是一种由多种微生物产生的可生物降解塑料。鉴于塑料所引发的新出现的环境问题,聚(3-羟基丁酸酯)作为传统塑料的替代品已受到关注。在本研究中,我们分离出了一种新型的产聚(3-羟基丁酸酯)微生物,即YI8菌株,它利用蔗糖作为生产聚(3-羟基丁酸酯)的经济高效碳源。在优化条件下,YI8菌株以蔗糖作为唯一碳源生产出了6.2克/升的聚(3-羟基丁酸酯)。从YI8中提取的聚(3-羟基丁酸酯)呈现出一种源自YI8天然产生的染料的粉红色。对由提取的聚(3-羟基丁酸酯)聚合物制成的薄膜进行了分析,包括凝胶渗透色谱法、万能试验机和差示扫描量热法,以确定其物理性质。所获得的值与从[具体菌株1]和H16中提取的聚(3-羟基丁酸酯)薄膜的值几乎相同。总体而言,本研究展现了YI8菌株作为产聚(3-羟基丁酸酯)菌株以及从该菌株中提取的聚(3-羟基丁酸酯)薄膜的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/d52d7e46eef6/polymers-17-01471-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/720143a3d5bf/polymers-17-01471-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/b493da930389/polymers-17-01471-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/de60957931ea/polymers-17-01471-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/a193a470872b/polymers-17-01471-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/43df81d79831/polymers-17-01471-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/d52d7e46eef6/polymers-17-01471-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/720143a3d5bf/polymers-17-01471-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/b493da930389/polymers-17-01471-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/de60957931ea/polymers-17-01471-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/a193a470872b/polymers-17-01471-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/43df81d79831/polymers-17-01471-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7040/12158085/d52d7e46eef6/polymers-17-01471-g006.jpg

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本文引用的文献

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Polyhydroxybutyrate production from crude glycerol using a highly robust bacterial strain Halomonas sp. YLGW01.利用高度健壮的细菌菌株盐单胞菌属YLGW01从粗甘油生产聚羟基丁酸酯。
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