Giles Tim, van Limbergen Tommy, Sakkas Panagiotis, Quinn Lily, Belkhiri Aouatif, Maes Dominiek, Kyriazakis Ilias, Barrow Paul, Foster Neil
University of Nottingham, UK.
Ghent University, Belgium.
Genomics. 2020 Sep;112(5):3218-3225. doi: 10.1016/j.ygeno.2020.03.010. Epub 2020 Mar 18.
Coccidiosis in broiler chickens, caused by infection with Eimeria spp. remains one of the most economically important production diseases. Development of a genetic biomarker panel of sub-clinical infection would be an important biological tool for the management of broiler flocks. We analysed expression of MicroRNAs (miRNAs) to determine the potential for these in diagnosing coccidiosis in broiler flocks. miRNA expression, in the ilea of Ross 308 broilers, was compared between chickens naturally clinically or sub-clinically infected with Eimeria maxima and Eimeria acervulina using NextSeq 500 sequencing. 50 miRNAs with greatest coefficient of variance were determined and principal component analysis showed that these miRNAs clustered within the clinical and sub-clinical groups much more closely than uninfected controls. Following false detection rate analysis and quantitative PCR we validated 3 miRNAs; Gallus gallus (gga)-miR-122-5p, gga-miR-205b and gga-miR-144-3p, which may be used to diagnose sub-clinical coccidiosis.
由艾美耳属感染引起的肉鸡球虫病仍然是最重要的经济生产疾病之一。开发亚临床感染的遗传生物标志物面板将是肉鸡群管理的重要生物学工具。我们分析了微小RNA（miRNA）的表达，以确定其在诊断肉鸡群球虫病方面的潜力。使用NextSeq 500测序技术，比较了自然临床感染或亚临床感染巨型艾美耳球虫和堆型艾美耳球虫的罗斯308肉鸡回肠中的miRNA表达。确定了方差系数最大的50种miRNA，主成分分析表明，这些miRNA在临床组和亚临床组中的聚集比未感染对照组更为紧密。经过错误检测率分析和定量PCR，我们验证了3种miRNA；鸡（gga）-miR-122-5p、gga-miR-205b和gga-miR-144-3p，它们可用于诊断亚临床球虫病。
