评价商业检测试剂 Policimbac®对多黏菌素 B 的药敏试验。
Evaluation of the susceptibility test of polymyxin B using the commercial test Policimbac®.
机构信息
Laboratório de Pesquisa em Resistência Bacteriana-LABRESIS, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
出版信息
Braz J Microbiol. 2020 Sep;51(3):1135-1137. doi: 10.1007/s42770-020-00246-9. Epub 2020 Mar 20.
Abstract
Broth microdilution (BMD), the reference method to determine bacterial susceptibility to polymyxins, is a laborious and time-consuming technique. Policimbac® is a commercial test panel which uses lyophilized polymyxin B to determine the minimum inhibitory concentration for Gram-negative isolates. This study evaluated the performance of Policimbac® in comparison with BMD for 110 isolates. Although the Policimbac® presented a very low essential agreement, the categorical agreement with BMD was optimal. Policimbac® is an alternative approach to BMD for evaluating the susceptibility to polymyxin B.
摘要
肉汤微量稀释法（BMD）是一种用于确定细菌对多粘菌素敏感性的参考方法，该方法繁琐且耗时。Policimbac®是一种商业测试板，它使用冻干的多粘菌素 B 来确定革兰氏阴性分离物的最小抑菌浓度。本研究评估了 Policimbac®与 BMD 对 110 株分离株的性能。尽管 Policimbac®的基本一致性非常低，但与 BMD 的分类一致性是最佳的。Policimbac®是评估多粘菌素 B 敏感性的 BMD 的替代方法。
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本文引用的文献
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Int J Antimicrob Agents. 2017 Aug;50(2):282-284. doi: 10.1016/j.ijantimicag.2017.05.003. Epub 2017 Jun 1.
2
Co-occurrence of mcr-1 and blaKPC-2 in a clinical isolate of Escherichia coli in Brazil.巴西一株临床分离大肠杆菌中mcr-1与blaKPC-2的共现。
J Antimicrob Chemother. 2017 Aug 1;72(8):2404-2406. doi: 10.1093/jac/dkx142.
3
Polymyxins: Antibacterial Activity, Susceptibility Testing, and Resistance Mechanisms Encoded by Plasmids or Chromosomes.多粘菌素：抗菌活性、药敏试验以及由质粒或染色体编码的耐药机制
Clin Microbiol Rev. 2017 Apr;30(2):557-596. doi: 10.1128/CMR.00064-16.
4
Resistance to polymyxins in Gram-negative organisms.革兰氏阴性菌对多黏菌素的耐药性。
Int J Antimicrob Agents. 2017 May;49(5):526-535. doi: 10.1016/j.ijantimicag.2016.11.029. Epub 2017 Feb 3.
5
Epidemiology of infections caused by polymyxin-resistant pathogens.多粘菌素耐药病原体引起的感染的流行病学。
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6
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Int J Infect Dis. 2014 Aug;25:79-81. doi: 10.1016/j.ijid.2014.01.005. Epub 2014 May 21.
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J Clin Microbiol. 2009 May;47(5):1611-2. doi: 10.1128/JCM.02466-08. Epub 2009 Mar 4.
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