Laboratório de Pesquisa em Resistência Bacteriana-LABRESIS, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Braz J Microbiol. 2020 Sep;51(3):1135-1137. doi: 10.1007/s42770-020-00246-9. Epub 2020 Mar 20.
Broth microdilution (BMD), the reference method to determine bacterial susceptibility to polymyxins, is a laborious and time-consuming technique. Policimbac® is a commercial test panel which uses lyophilized polymyxin B to determine the minimum inhibitory concentration for Gram-negative isolates. This study evaluated the performance of Policimbac® in comparison with BMD for 110 isolates. Although the Policimbac® presented a very low essential agreement, the categorical agreement with BMD was optimal. Policimbac® is an alternative approach to BMD for evaluating the susceptibility to polymyxin B.
肉汤微量稀释法（BMD）是一种用于确定细菌对多粘菌素敏感性的参考方法，该方法繁琐且耗时。Policimbac®是一种商业测试板，它使用冻干的多粘菌素 B 来确定革兰氏阴性分离物的最小抑菌浓度。本研究评估了 Policimbac®与 BMD 对 110 株分离株的性能。尽管 Policimbac®的基本一致性非常低，但与 BMD 的分类一致性是最佳的。Policimbac®是评估多粘菌素 B 敏感性的 BMD 的替代方法。
