Department of Applied Chemistry, SV National Institute Technology, Surat 395007, Gujarat, India.
Department of Chemistry, ACC Wing, IMA Dehradun, Uttarakhand 248007, India.
Food Chem. 2020 Aug 1;320:126611. doi: 10.1016/j.foodchem.2020.126611. Epub 2020 Mar 14.
Histamine, one of the most important biogenic amines (BAs) is considered as food hazard and therefore various agencies have fixed threshold in different food and beverages. In this manuscript, two novel fluorescent turn-on probes were developed for the instantaneous detection of histamine. The β-cyclodextrin (β-CD) capped ZnO quantum dots (QDs) were decorated with the vitamin B cofactors like pyridoxal 5'-phosphate (PLP) and pyridoxal (Py) by forming host-guest inclusion complexation between the capped β-CD and PLP/Py. The cofactors decorated QDs (ZnO@PLP and ZnO@Py) were applied for the sensing of BAs. Addition of histamine to the ZnO@PLP and ZnO@Py solution resulted selective fluorescence enhancement at 473 nm and 460 nm, respectively. Without any interference from the other tested BAs, the fluorescence response of the probes ZnO@PLP and ZnO@Py showed good linearity to histidine concentration from 2.49 to 24.4 μM and 7.44 to 47.6 μM with the detection limit down to 0.59 μM and 0.97 μM, respectively.
组氨酸是最重要的生物胺（BAs）之一，被认为是食物危害，因此各个机构都在不同的食品和饮料中设定了阈值。在本文中，开发了两种新型荧光开启探针，用于即时检测组氨酸。β-环糊精（β-CD）封端的氧化锌量子点（QDs）通过形成封端β-CD 与 PLP/Py 之间的主体-客体包合络合物，用维生素 B 辅因子如吡哆醛 5'-磷酸（PLP）和吡哆醛（Py）进行修饰。修饰后的辅因子 QDs（ZnO@PLP 和 ZnO@Py）用于检测 BAs。向 ZnO@PLP 和 ZnO@Py 溶液中加入组氨酸，分别在 473nm 和 460nm 处产生选择性荧光增强。在没有其他测试的 BAs 干扰的情况下，探针 ZnO@PLP 和 ZnO@Py 的荧光响应对组氨酸浓度表现出良好的线性关系，从 2.49 到 24.4μM 和从 7.44 到 47.6μM，检测限分别低至 0.59μM 和 0.97μM。
