Malya Irine Yunhafita, Wu Jing, Harada Etsuko, Toda Masaaki, D'Alessandro-Gabazza Corina N, Yasuma Taro, Gabazza Esteban C, Choi Jae-Hoon, Hirai Hirofumi, Kawagishi Hirokazu
Graduate School of Science and Technology, Shizuoka University , Shizuoka, Japan.
Research Institute of Green Science and Technology, Shizuoka University , Shizuoka, Japan.
Biosci Biotechnol Biochem. 2020 Jul;84(7):1332-1338. doi: 10.1080/09168451.2020.1743170. Epub 2020 Mar 21.
A novel compound, -4-ethoxy-2-hydroxy-4-oxobutanoic acid (), and six known compounds (-) were isolated from the fruiting bodies of the wild edible mushroom . The planar structure of was determined by the interpretation of spectroscopic data analysis. The absolute configuration of was determined by comparing specific rotation of the synthetic compounds. In the plant regulatory assay, the isolated compounds (-) and the chemically prepared compounds (-) were evaluated their biological activity against the lettuce () growth. Compounds and - showed the significant regulatory activity of lettuce growth. showed the strongest inhibition activity among the all the compounds tested. In the lung cancer assay, all the compounds were assessed the mRNA expression of Axl and immune checkpoints (PD-L1, PD-L2) in the human A549 alveolar epithelial cell line by RT-PCR. Compounds - showed significant inhibition activity against Axl and/or immune checkpoint.
从野生可食用蘑菇的子实体中分离出一种新型化合物——4-乙氧基-2-羟基-4-氧代丁酸（ ）以及六种已知化合物（ ）。通过光谱数据分析的解读确定了 的平面结构。通过比较合成化合物的比旋光度确定了 的绝对构型。在植物调节试验中，对分离出的化合物（ ）和化学合成的化合物（ ）评估了它们对生菜（ ）生长的生物活性。化合物 和 显示出对生菜生长的显著调节活性。在所有测试化合物中， 显示出最强的抑制活性。在肺癌试验中，通过逆转录聚合酶链反应（RT-PCR）评估了所有化合物对人A549肺泡上皮细胞系中Axl和免疫检查点（PD-L1、PD-L2）的mRNA表达的影响。化合物 对Axl和/或免疫检查点显示出显著的抑制活性。
